Plant Defense Gene Expression Analysis

Plant defense gene research is an important research direction in botany, which helps to reduce crop disease losses, improve crop yield and economic benefits. Creative BioMart Microbe provides a complete set of solutions for plant defense gene expression, and have established a one-stop service platform from nucleic acid extraction to data statistical analysis.

Plant Defense Genes

As sessile organisms, plants are attacked by pathogenic microorganisms such as viruses, bacteria, and fungi during their growth. Plants have developed a set of defense responses to infection by pathogenic microorganisms, including deposition of cell wall material, accumulation of disease-related hydrolases (eg, chitinase and glucanases), and synthesis of phytoprotectins and protease inhibitors. Plant defense genes refer to a class of genes evolved in plants in response to pathogenic microorganisms. They can express proteins involved in the defense response of plants to pathogenic microorganisms under the induction of pathogenic microorganisms.

Plant Defense Gene Expression Analysis

A variety of analysis methods can be used to analyze the expression of plant defense genes, including differential expression analysis, gene co-expression analysis, differentially expressed genes (DEGs) identification, KEGG pathway enrichment analysis, GO enrichment analysis, etc.

• Detection of DEGs during plant immune responses is an efficient method for screening plant defense gene candidates. The difference in expression levels of genes in two samples and the statistical significance of the difference can be quickly analyzed by volcano plots.
• Genes in the same pathway tend to co-express in expression values. Gene co-expression analysis uses a large amount of gene expression data to construct correlations between genes to explore gene functions.
• According to the classification of the GO database, genes are divided into three categories: cellular component (CC), molecular function (MF), and biological process (BP). The use of GO enrichment analysis is helpful to mine the functions and signaling pathways of plant defense genes.
• KEGG pathway enrichment analysis studies genes and their expression information as a whole network. Systematic analysis of gene products by enrichment analysis helps to understand the metabolic pathways and functions of defense genes in cells.

Figure 1. Analysis of the DEGs following wounding in S. tora leaves (Kang, et al. 2021)

Plant Defense Gene Analysis Process

Why Choose Creative BioMart Microbe?

• Creative BioMart Microbe has established a one-stop plant defense gene expression analysis platform from sample preprocessing, RNA extraction, RNA sequencing to data analysis, providing customers with efficient analysis services.
• We have a team of bioinformatics experts who conduct comprehensive and professional analysis of sequencing data and provide customers with authoritative analysis reports.
• Cost-effective services help advance botany research, and timely service feedback brings satisfactory experience to customers.

If you want to know more information about this service, please feel free to contact us by phone or email, or you can consult through our inquiry form.

Reference

1. Kang, J.N., et al. Systemic Expression of Genes Involved in the Plant Defense Response Induced by Wounding in Senna tora. Int. J. Mol. Sci. 2021, 22(18): 10073.