Large-Scale Protein Production

Summary of the Recombinant Protein Expression and Purification

Large-scale recombinant protein production is becoming more and more important for applications in proteomics, structural biology, and precision medicine. In the process of drug development, research on the characterization and function of proteins can provide important clues about the mode of action, thereby greatly accelerating the development process. For example, through structural biology-related data, it is possible to design inhibitors that bind to disease-related enzymes.

However, to successfully study protein structure and function, large quantities of protein (mg to g amount) must be expressed and isolated from an appropriate expression system. In order to meet this demand, cell culture needs to be cultured in large quantities through the implementation of fermentation technology. Fermentation improves efficiency by increasing cell density and provides large cultures over 4 liters that are easy to handle. According to high cell density and fermentation strategy, we can optimize cell culture conditions to increase cell quality and increase recombinant protein production. Of course, fermentation conditions are greatly affected by various parameters in the fermentation process. By optimizing fermentation conditions and purification strategies, we can increase cell quality and ultimately increase recombinant protein production.

Large-Scale Protein Production Provided by Creative BioMart Microbe

Creative BioMart Microbe is a biotechnology company composed of an experienced professional team. We have introduced many equipment to meet the needs of large quantities of pure and active proteins and large-scale protein production and purification services under GLP. Our excellent protein expression platform is equipped with a series of bioreactors (including fermenters and wave mixers, etc.) to meet the needs of protein production of different scales, ranging from milligrams to hundreds of grams. For example, our bacterial expression platform, coupled with our large-scale protein production platform, can achieve a bacterial fermentation culture of up to 2000 L, and provide recombinant proteins with purity of over 98% for crystallization (partially purified recombinant protein with purity of over 70%).

Creative BioMart Microbe has accumulated nearly 20 years of experience in this field. Our optimized fermentation conditions and purification strategies can help customers solve the difficulties and challenges encountered in protein expression and purification in all aspects.

Advantages of Our Service

• A variety of expression systems to choose from
• Customize the optimal solution for different proteins
• Combination of multiple technology platforms
• One-stop service
• Cost-effective

If you want to know more about this service, please feel free to contact us by phone or email, or you can consult through our inquiry form. We will ensure to provide you with efficient and high-quality experimental outsourcing services.