Background

Overview of Environmental Protection Microbiological Agents

Environmental protection microbial agents are a class of microbial products used for environmental governance and ecological protection, and they play an important role in pollution detection, treatment and remediation of water, air, soil and solid waste. These microbial inoculants can be isolated and purified from nature, or one or more microbial strains obtained through artificial breeding by modern biotechnology means.

Environmental protection microbial agents have a wide range of applications, including but not limited to wastewater treatment, soil remediation, solid waste treatment, etc. In wastewater treatment, aerobic bacteria and anaerobic bacteria can effectively degrade organic matter and improve treatment efficiency. With the increasingly prominent global environmental problems, the application and development trend of environmental protection microbial agent as a new environmental protection technology has attracted much attention.

Fig. 1. Prospective technologies map for oil pollutants biodegradations by microorganisms. (Xu, et al., 2023)

Classification of Environmental Protection Microbial Agents

The classification of environmental protection microbial agents can be divided from different perspectives, mainly based on their mechanism of action, field of application, and target pollutants. General classification according to the application field can be divided into the following categories:

• Organic waste treatment agents: Used to treat organic waste generated by agriculture, animal husbandry and food processing industry and promote its degradation into organic fertilizer.
• Soil remediation agents: Used to repair contaminated soil, decompose and adsorb toxic substances in soil, and improve soil structure.
• Water purification agents: Used to treat polluted rivers, lakes and other water bodies, decomposition and adsorption of organic matter and heavy metal ions in water.
• Air purification agents: Used to improve air quality, absorb and decompose harmful substances in the air.

The development trend of environmental protection microbial agents shows that the future industry will pay more attention to the innovation of biotechnology and the improvement of product efficiency. The application of microbial inoculants will be more precise and customized to meet the needs of different geographical environments and crop types. At the same time, the contribution of microbial agents in green agriculture, circular economy, environmental pollution control and other aspects will be increasingly large, and become an important support for sustainable development.

Creative BioMart Microbe, with its expertise and experience, is committed to providing society with a high-quality range of environmental protection microbial agents, in addition to supporting the customized products by production service. Please feel free to contact us for more information.

Service Procedure

Service Details

Microorganism Isolation in Samples

It is expected to isolate some efficient degrading strains from the existing polluted environment. Creative BioMart Microbe has a professional microbiology testing laboratory to perform microbiological testing services of different samples. And we also rich experience in microbiome analysis, and has established advanced microbiome analysis platforms, including GeoChip, metagenomics sequencing, metatranscriptomics sequencing, and microbial metabolomics analysis technology platforms.

High-yield Strain Screening

Rapid identification and screening of high yield target product characteristics from a large number of microbial strains can improve production efficiency and reduce costs. Creative BioMart Microbe strives to serve clients by providing strain improvement service with a panel of comprehensive solutions that meet the highest industry and production expectations. We offer a variety of strain improvement services including Mutation Service, Random Mutagenesis Service, Genetic Recombination Service, Overexpression Service and Gene Editing Service. You can choose the right service for your needs.

Fermentation Process Optimization

Our experts have many years of service experience in the field of microbial fermentation, and have the professional ability to help customers develop fermentation processes, including batch fermentation, continuous fermentation and fed-batch fermentation. With the integration of the high-throughput bioreactor system, we can help researchers to swiftly carry out intricate experiments for the purpose of discovering novel protocols or refining current methods.

Customized Fermentation Production

Our team, with extensive expertise in microbial fermentation, possesses the specialized skills to assist clients in crafting tailored fermentation strategies. This encompasses a range of processes from batch to continuous and fed-batch fermentation. Additionally, we extend our services to include the fine-tuning of fermentation operations, focusing on critical parameters such as the composition of the culture medium, the volume of inoculum, temperature control, pH levels, dissolved oxygen levels, and the modalities of feeding. Our goal is to streamline our clients' manufacturing processes to enhance efficiency and productivity.

Optimization of Freeze-drying Process

Depending on the microbial activity and characteristics, we will obtain microbial products in different formats, which may be powder or liquid. For powder agents, we can optimize the freeze-drying process. The freeze-drying process consists of three stages: (1) freezing stage, (2) primary drying stage and (3) secondary drying stage. The key parameters in the freeze-drying test under various conditions were optimized to shorten the cycle of the freeze-drying process without affecting the quality of the freeze-drying products (ensuring low moisture content and excellent product appearance).

Our Advantages

• Abundant raw materials: We can provide a variety of application areas of microbial species, including nitrifying bacteria, denitrifying bacteria, lactic acid bacteria, acetic acid bacteria, fungi such as mold and yeast, as well as some plant extracts and fermentation products.
• Extensive fermentation experience: We specialize in microbial fermentation and have many years of industry experience.
• Flexible scale production: We have the ability to scale from laboratory scale to small pilot runs to mass production.

Case Study

Case Study 1: High-efficiency aerobic denitrifier Bacillus megaterium S379 for aquaculture wastewater treatment.

Excessive accumulation of nitrite is an extremely difficult problem for the intensive aquaculture industry. To address this challenge, the researchers successfully selected a highly efficient aerobic denitrification bacterium, Bacillus megaterium S379, which can achieve a nitrite nitrogen removal rate of up to 91.71%±0.17% (65 mg/L). The S379 demonstrates excellent environmental adaptability, maintaining a nitrite removal rate of more than 85% at temperatures between 25°C and 40°C and pH between 7.0 and 9.0, and can withstand nitrite nitrogen concentrations up to 560 mg/L. When the amount of nitrite nitrogen added reached 340 mg/L, the denitrification performance of S379 was further enhanced by immobilization technology. The immobilized cells also showed a good pollutant removal effect in the treatment of aquaculture wastewater.

Fig. 2. Nitrite removal capability of immobilized S379 under different NO₂--N content. (Gao, et al., 2018)

Case Study 2: The bioremediation strategy using Sedum alfredii and DDT-degrading microbes effectively reduced the content of cadmium and DDT metabolites in co-polluted soil.

The aim of this study was to develop a bioreremediation strategy for soils contaminated by cadmium (Cd) and DDT and its metabolites (DDE and DDD, collectively DDs) using the Cd-hyperenriched plant Sedum alfredii (SA) and DDT-degrading strain (DT-1). In the pot experiment, DT-1 inoculation first increased the root biomass of SA. The 18-month pot experiment showed that the combined application of SA and DDT-1 reduced the contents of Cd and DDs in contaminated soil by 32.1-40.3% and 33.9-37.6%, respectively, while the non-planted and untreated controls only reduced the contents by 3.25% and 3.76%, respectively. Subsequent field experiments (lasting 18 months) further confirmed the effectiveness of the method, and the content of Cd and DDs decreased by 31.1% and 53.6%, respectively.

Fig. 3. Removal efficiencies of Cd and DDs by SA with or without DDT-degrading microbes (M). (Zhu, et al., 2012)

Case Study 3: Development of a new efficient microbial preparation for kitchen waste degradation "YH".

In this study, 12 highly degradable strains were selected from kitchen waste and a new composite microbial preparation "YH" was developed. YH" is superior to commercially available microbial agents in terms of reduction, maturity and deodorization, with a mass loss rate of 65.87% within 14 days, significantly reducing NH3 and H2S emissions, and the decrease of E4/E6 values indicates that it has a significant effect on promoting compost maturation.

Fig. 4. Degradation characteristics of 12 microbial strains. (Zhao, et al., 2017)

FAQs

References:

1. Xu CY.; et al. The role of microorganisms in petroleum degradation: Current development and prospects. Sci Total Environ. 2023;865:161112.
2. Gao J.; et al. Biopotentiality of High Efficient Aerobic Denitrifier Bacillus megaterium S379 for Intensive Aquaculture Water Quality Management. J Environ Manage. 2018;222:104-111.
3. Zhu ZQ.; et al. Bioremediation of Cd-DDT co-contaminated soil using the Cd-hyperaccumulator Sedum alfredii and DDT-degrading microbes. J Hazard Mater. 2012;235-236:144-151.
4. Zhao K.; et al. Development of a novel compound microbial agent for degradation of kitchen waste. Braz J Microbiol. 2017;48(3):442-450.