Disinfectant Testing for Contact Lenses Solution

The contact lens solution is used to disinfect contact lenses and protect the eye health of contact lens users. In order to ensure the ability of contact lens solution to kill pathogens, the International Organization for Standardization specifies the evaluation test method for the disinfection ability of contact lens solution. Creative BioMart Microbe provides customers with testing services for contact lens solutions based on this standard. We can also design personalized contact lens solution testing solutions based on the specific needs of customers.

The soft texture of contact lenses can provide an ideal breeding environment for pathogens. Many pathogens may be introduced during the wearing of contact lenses, the introduction of these pathogens can cause eye infections, such as bacterial keratitis and bacterial conjunctivitis. The contact lens solution is an aqueous solution containing antibacterial agents, which can reduce the microbial contamination introduced in the process of wearing, removing, cleaning and storing contact lenses. In order to ensure that the contact lens solution products comply with international standards, it is necessary to conduct relevant antibacterial ability tests on these products during the product development stage to ensure that these products have appropriate antibacterial activity.

Test Organisms

Test Process

• Prepare a suspension of test microorganisms. After appropriate dilution, the turbidity of the suspension is measured with a spectrophotometer to estimate the cell concentration, or the actual concentration can be determined by the plate count method.
• Stand-alone procedure

Prepare the test product solution and inoculate the test microorganisms. According to the 25%, 50%, 75%, and 100% of the minimum recommended disinfecting time, the viable count of the inoculated product is determined. For molds and yeasts, it is not less than 400% of the minimum recommended disinfecting time. Take out aliquots at specified time intervals and properly dilute them with neutralizing media. After the neutralization is completed, incubate the microbial recovery plates at an appropriate temperature (the incubation time for microbial recovery plates should be determined by testing), record the number of colonies on the countable plate, and calculate the microbial reduction at the specified time points.

• Regimen procedure

Place the test lens and the control lens in a sterile petri dish (with the concave surface facing up). Place 0.01 ml of the inoculum on the contact point between the petri dish and each lens, and inoculate 0.01 ml of the same inoculum on the concave surface of the same lens. After the absorption of the inoculum, the contact lenses are cleaned, rinsed, and soaked according to the instructions. Add a neutralizing medium to the filtration apparatus, transfer the lens and solution into the neutralizing medium, filter under reduced pressure, and rinse the filter with the neutralizing medium. Transfer the contact lens to the agar plate used for the recovery of the test microorganisms, and pour the same culture medium over the lens. The test filter is also placed on an agar plate. Incubate the microbial recovery plates at an appropriate temperature, and record the incubation result.

Workflow of Contact Lenses Solution Testing Service at Creative BioMart Microbe

Creative BioMart Microbe can provide you with efficient and high-quality contact lens solution testing services. If you want to know more about this service, please feel free to contact us by phone or email, or you can consult through our inquiry form. We will ensure to provide you with efficient and high-quality experimental outsourcing services.