Antigen Preparation Service
Antigen is a substance capable of triggering an effective immune system response. Selecting the proper antigen is one of the determining factors in custom antibody development. Nearly any kind of antigen can be used for antibody production, like proteins, peptides, SDS-Gel pieces, DNA, cells, virus particles or small molecules like toxins, haptens or drugs.
Peptides are the specific option for certain epitope-specific antibodies like phospho-specific antibodies. Our peptides preparation services include peptide design, peptide synthesis, peptide conjugation and other custom services.
Full length native proteins, soluble active protein domains even or intact membrane protein are usually the antigens of choice and the preferred target for high-quality antibody generation. We can choose scientifically the suitable protein regions, use bioinformatics tools to find best sequences, and synthetize gene optimized DNA fragment for protein production.
Whole cells can also be employed as antigen for antibody development. Whole cell antigen can cause higher immunogenicity and avoid complex purification process, which is an alternative option for the development of anti-membrane protein antibody. We can culture the provided cell lines for specific project.
Hapten antigens are small molecules like aniline, urushiol, hydralazine, and digoxigenin that are antigenic but not immunogenic. Specific antibody against a hapten can only be raised through properly designed hapten-carrier conjugation and adjuvant.
The antigen types Creative BioMart Microbe offers:
- Peptide and conjugates antigen production
- Native protein antigen production
- Recombinant protein antigen production
- DNA and small molecules antigen production
- Others…
Advantages of our antigen preparation service:
- Best immunogenic domain design.
- Different alternative tags: GST tag, His tag, MBP tag…
- Multiple recombinant protein production systems: prokaryotic expression, eukaryotic expression, insect and baculovirus expression, mammalian expression.
- Various purification technology: protein precipitation technology, affinity chromatography, size exclusion chromatography, Ion exchange chromatography, hydrophobic interaction and others…
Creative BioMart Microbe is a leading provider of comprehensive, high quality antigen preparation service supplier. If you are seeking antigen preparation service, Creative BioMart Microbe is an excellent choice. Please feel free to contact us for more information.
