MultiLocus Sequence Typing (MLST)
Multilocus sequence typing (MLST) is a typing method derived from multilocus enzyme electrophoresis (MLEE), which is widely used in the typing of bacteria, epidemiology and microbial evolutiology. MLST is a technology for strain typing by directly determining the nucleotide sequence of multiple housekeeping genes, which can classify the same species of bacteria into more subtypes.
Principle of MultiLocus Sequence Typing (MLST)
There are some housekeeping genes in bacteria, and these gene fragments are of different lengths and have different polymorphisms in different strains. MLST is a bacterial typing method based on the comparison of nucleotide sequences. It usually compares the polymorphism of the nucleotide sequence of the housekeeping genes within the bacteria (generally 7 housekeeping genes are selected). Design primers for housekeeping genes, and amplify the nucleotide sequence of housekeeping genes in bacteria by PCR. By sequencing the amplified product and comparing it with the corresponding gene in the database, an allelic profile can be obtained and the sequence type (ST) of the strain can be determined. Each ST represents a set of individual nucleotide sequence information, and the correlation between strains can be found by comparing the ST of the strains, so as to achieve the purpose of strain typing.
The use of MLST technology can realize the ST analysis of strains, as well as phylogenetic tree analysis and clone complex analysis. MLST can be used for typing up to 100 kinds of bacteria, applicable strains include Bacillus cereus, Bacillus licheniformis, Listeria monocytogenes, Propionibacterium acnes, Salmonella enterica, Campylobacter spp, Cronobacter spp, Neisseria meningitidis, Staphylococcus aureus, Streptococcus pyogenes, Candida albicans, Escherichia coli, etc.
Technical advantages
- High discriminatory power
- Easy to operate, good repeatability, short experiment period and low cost
- Classify the same species of bacteria into more subtypes
- Easily compared with the allelic profiles in the database
Technical Process
Our MLST Service May Help You in The Following Areas (including but not limited to the following):
- Epidemiological study of bacteria. Conducive to the identification of pathogens of outbreaks and monitoring of epidemics.
- Microbial evolutiology study. It can be used to determine the phylogenetic relationship between different ST types, and is conducive to the investigation of flora structure and the analysis of flora genetics
Workflow of MLST Service at Creative BioMart Microbe
* You need to provide a DNA sample of the strain to be tested (total amount> 1 μg, concentration ≥ 10 ng/μl, if we need to purify the sample, you need to provide more than 2 μg DNA and additional instructions when submitting the sample) or bacterial liquid (the DNA extraction step will be carried out by our technical experts). If you have selected primers, please provide primer-related information.
* We will deliver to you a detailed experiment report, including experiment procedures, data, and professional analysis reports.
Creative BioMart Microbe provides customers with microbiological-related technical services, a professional technical team and a strict quality management system to ensure that we provide customers with accurate and reliable experimental results.
If you want to know more about this service, please feel free to contact us by phone or email, or you can consult through our inquiry form. We will ensure to provide you with efficient and high-quality experimental outsourcing services.
