Phage Formulation Development Services
BackgroundService ProcedureOur AdvantagesCase StudyFAQs
Background
As bacteriophage therapy re-emerges as a promising strategy to combat multidrug resistant infections and support microbiome-targeted applications, the development of stable and effective phage formulations has become a critical component in translating phage-based products from bench to bedside. Unlike small molecule drugs, bacteriophages are biological entities that are highly sensitive to environmental conditions such as pH, temperature, ionic strength, and desiccation. Their structural complexity and diversity require tailored formulation strategies to preserve infectivity, ensure shelf stability, facilitate targeted delivery, and meet regulatory requirements for safety and consistency.
Creative BioMart Microbe offers a comprehensive range of phage formulation development services to optimize the stability, delivery and efficacy of bacteriophages for various applications. Our expertise includes phage dosage form design services, agricultural phage development services, industrial phage development services, and functional phage product development services to ensure that each phage formulation is tailored to meet the specific requirements of therapeutic, agricultural, and industrial applications. Contact us today for more information!
Service Procedure
Services Details
Phage Dosage Form Design Services
We develop customized phage dosage forms for oral, topical, injectable and inhalation routes. Our formulations optimize phage stability, bioavailability, and therapeutic efficacy to support clinical development and regulatory compliance. From early-stage concepts to scalable prototypes, we provide solutions that transform phage formulations into patient-ready medicines.
Agricultural Phage Development Services
Our agricultural phage solutions target plant and livestock pathogens with eco-friendly, residue-free alternatives to antibiotics and chemicals. We develop phage-based products for crop protection, soil health, and animal husbandry, ensuring efficacy, environmental safety and regulatory compliance to support sustainable agricultural practices.
Industrial Phage Development Services
We offer tailored phage solutions for industrial applications such as food safety, fermentation, biocontrol, and bioremediation. From contamination prevention to targeted microbial management, our services enable safer, cleaner, and more efficient processes across food, beverage, pharma, and biotech manufacturing environments.
Phage Functional Product Development Services
We develop functional phage-based products for the health, hygiene and wellness markets. From probiotics and oral care to wound care and disinfectants, our team formulates innovative, bioactive products that leverage phage specificity to deliver targeted microbial control with consumer stability and appeal.
Our Advantages
- Customized Formulation Expertise: Tailored approaches for each phage type and intended use.
- Multifunctional Delivery Support: Solutions for oral, topical, aerosolized, and injectable phage products.
- Stability Optimization: Proven strategies to maximize shelf life and cold chain flexibility.
- Cross-functional Integration: In-house synergy with our phage purification, QC, and production services.
- Regulatory Preparedness: Formulations developed with downstream regulatory requirements in mind.
Case Study
Case Study 1: Phage cocktail powder for Pseudomonas aeruginosa respiratory infections.
A phage cocktail powder targeting multidrug-resistant Pseudomonas aeruginosa was developed by spray-drying three phages (PEV1, PEV2, and PEV20) with lactose and leucine as excipients. The resulting formulation-maintained phage viability with minimal titer loss, exhibited aerodynamic properties suitable for inhalation, and demonstrated broad-spectrum efficacy in vitro. Compared to single phage approaches, this cocktail improves therapeutic coverage and helps mitigate bacterial resistance, offering a promising strategy for inhaled phage therapy of pulmonary infections.
Figure 1. Titer of PEV1, PEV2 and PEV20 against three bacterial isolates P. aeruginosa JIP 62, JIP 117, and JIP 125. (Li et al., 2021)
Case Study 2: Formulation and humidity effects on spray-dried phages for Acinetobacter baumannii respiratory infections.
This study explored the use of an anti-Acinetobacter baumannii phage (vB_AbaM-IME-AB406) in inhalable dry powders with excipients such as trehalose, mannitol and L-leucine. The effect of formulation composition and excipient concentration on phage stability, aerosol performance and storage were evaluated. Higher mannitol content improved particle dispersibility and storage stability, with minimal titer loss over 6 months. However, high humidity conditions (65% RH) significantly affected lung dose delivery. The study highlights the importance of considering environmental factors when optimizing phage powder formulations for global use.
Figure 2. Impact of excipients and humidity on stability and aerosol performance of spray-dried phage powders targeting A. baumannii respiratory infections. (Yan et al., 2021)
FAQs
Q: Can you formulate phage cocktails?
A: Yes, we develop and test formulations for both single-phage and multi-phage cocktails, ensuring compatibility and stability across all components.
Q: How do you assess formulation stability?
A: We use both accelerated and real-time ICH-compliant stability studies, including titer retention, pH monitoring, and physical inspection under various storage conditions.
Q: Do you offer lyophilized phage formulation development?
A: Yes. We develop dry formulations including lyophilized and spray-dried phages with optimized protectants to ensure long-term viability and reconstitution fidelity.
Q: Do you offer customized excipient screening and compatibility testing?
A: Yes. We perform excipient selection and compatibility testing based on your phage type, target application, and delivery route to ensure maximum activity retention and product safety.
Q: Can you help co-formulate phages with antibiotics, enzymes, or prebiotics?
A: Yes. We provide co-formulation services for phages with synergistic agents, ensuring compatibility and enhanced therapeutic performance, especially for multidrug-resistant infection.
Q: How do I start a formulation project with you?
A: Simply contact our team to discuss your phage candidates, intended application, and delivery goals. We'll guide you through our process from concept to formulation prototype.
References:
- Kaur G, Agarwal R, Sharma RK. Bacteriophage therapy for critical and high-priority antibiotic-resistant bacteria and phage cocktail-antibiotic formulation perspective. Food Environ Virol. 2021;13(4):433-446. doi:10.1007/s12560-021-09483-z
- Li M, Chang RYK, Lin Y, Morales S, Kutter E, Chan HK. Phage cocktail powder for Pseudomonas aeruginosa respiratory infections. International Journal of Pharmaceutics. 2021;596:120200. doi:10.1016/j.ijpharm.2021.120200
- Yan W, He R, Tang X, et al. The influence of formulation components and environmental humidity on spray-dried phage powders for treatment of respiratory infections caused by Acinetobacter baumannii. Pharmaceutics. 2021;13(8):1162. doi:10.3390/pharmaceutics13081162
Related Services:
