18S/ITS Sequencing Service

18S rRNA gene sequencing and internal transcribed spacer (ITS) sequencing are sequencing technologies commonly used in fungi and yeast. Creative BioMart Microbe has established a high-quality 18S/ITS sequencing technology platform, and has an experienced sequencing technology team and many outstanding bioinformatics experts, able to provide customers with efficient and reliable 18S/ITS sequencing services.

18S rRNA Gene Sequencing

18S rRNA gene is a DNA sequence of about 1800 bp that encodes the small ribosomal subunit rRNA of eukaryotes, which is composed of conserved regions and variable regions. The 18S rRNA gene is conservative in the biological evolution process, so the sequence analysis of its sequence can be used to study the genetic relationship of biological evolution. The sequencing of variable regions can identify unknown fungi and yeast species, and can also be used to study the diversity of microbial communities.

ITS Sequencing

There are two ITSs in eukaryotes, of which ITS1 is located between 18S and 5.8S rRNA genes, and ITS2 is located between 5.8S and 28S rRNA genes. The size of ITS is smaller than that of 18S rRNA gene and it is easy to analyze. Sequencing of ITS1 or ITS2 can distinguish and identify strains with relatively close relationships, and can also be used to analyze the diversity of fungal community structure in microbial communities.

Advantages of 18S/ITS Sequencing Technology for Fungi and Yeast Identification

• Compared with traditional identification methods, it has faster identification efficiency and can provide more accurate identification results
• Automated analysis process
• Low sample requirements and lower cost compared with metagenomic sequencing
• Multiple applications: species identification, microbial community diversity analysis, species composition difference analysis

Why Choose Creative BioMart Microbe?

• High-quality 18S/ITS sequencing technology platform and experienced sequencing technicians
• Suitable for samples from multiple sources such as feces, dental plaque, saliva, dander, soil, water, etc.
• Standardized operating procedures and complete quality management and guarantee systems can ensure accurate and reliable sequencing results for customers
• Excellent bioinformatics experts can provide customers with authoritative and professional microbial identification reports or microbial diversity analysis reports
• High-quality customer service, we guarantee 24-hour online service to ensure timely response to customers
• Shorter turnaround time and available urgent service

Sample Requirements

• Genomic DNA: The total sample volume is required to be ≥ 20 μL, and the genomic DNA concentration ≥ 10 ng/μL
• Plate or slope samples, or broths samples of pure culture: Plate and slope samples are required to have a single colony
• We also accept mixed cultures. Our strain isolation and purification services can help, but the delivery time of identification results may need to be extended

Workflow of 18S/ITS Sequencing Service

If you want to know more related service information, you can contact us by phone or email, or you can get in touch through our inquiry form.