Metabolite Content Determination

Microbial metabolites have broad research prospects in many fields such as nutrition, healthcare, and agriculture. Creative BioMart Microbe provides customers with a number of services related to microbial metabolites, including the determination of metabolite content. We facilitate our clients' personalized research solutions through a variety of alternative technology platforms.

Metabolites in Microorganisms

Microorganisms have a variety of metabolic activities, and their normal operation ensures that microorganisms can tolerate different chemical environments. Microorganisms produce a variety of metabolites during the metabolic process, including primary metabolites and secondary metabolites. Primary metabolism is a metabolic activity closely related to the growth and reproduction of organisms, and primary metabolites are substances necessary for microbial self-reproduction produced through metabolic activities, such as amino acids, nucleotides, polysaccharides, lipids, etc. The types of primary metabolites of different types of microorganisms are basically the same. Any disorder in the synthesis of primary metabolites will affect the normal life activities of microorganisms.

Secondary metabolism refers to the process in which microorganisms use primary metabolites as precursors to synthesize some substances that have no obvious physiological function for the microorganism and are not necessary for its growth and reproduction during a certain growth period. Disorders of secondary metabolism do not lead to cessation of microbial growth or death. The secondary metabolites of microorganisms include antibiotics, hormones, pigments, toxins, etc. The secondary metabolites of different types of microorganisms are different. Microbial secondary metabolites usually have complex chemical structures and are of great value in pharmaceutical research. Commonly used antibiotics such as streptomycin, penicillin, erythromycin and tetracycline are secondary metabolites of microorganisms.

Creative BioMart Microbe provides quantitative analysis and abundance determination services for different kinds of metabolites. We analyze metabolites based on multiple platforms, including liquid chromatography-mass spectrometry (LC-MS), gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography (HPLC), etc. We also provide refractive index detection, UV detection, enzymatic analysis, the Folin–Ciocalteu method, the aluminum chloride colorimetric method, etc. for the determination of metabolites.

• LC-MS is commonly used for the analysis of water-soluble small molecules such as amino acids, sugars, alcohols, organic acids, amines, and lipid macromolecules.
• GC-MS is commonly used for the analysis of water-soluble metabolites, some lipids and organic acids. We also provide biochemical analysis services for metabolites.

Why Choose Creative BioMart Microbe?

• Comprehensive metabolite services provide customers with one-stop solutions to shorten the research cycle
• Multiple technology platforms to meet different research needs
• Experienced technical team and strict quality management system ensure accurate and reliable test results
• Competitive pricing helps reduce research costs
• Realize real-time technical communication and tracking services.

Workflow of Metabolite Content Determination Service

Creative BioMart Microbe provides comprehensive microbial metabolite solutions, including metabolite extraction and isolation, identification, content determination, and metabolomics analysis services. Please feel free to contact us for more detailed service information, and our experts will communicate with you about your research project in time.