Pulsed-Field Gel Electrophoresis (PFGE)
Pulsed-Field Gel Electrophoresis (PFGE) is a mature molecular biological bacterial typing technology method. This method has the advantages of good typeability, good reproducibility, strong discriminating power, and has standardized technical solutions. It is considered as the "gold standard" for bacterial typing. PFGE separates DNA molecules of different sizes by changing the direction of the electric field and the appropriate pulse time (suitable for the separation of large molecular DNA, which can separate DNA molecules up to 10Mb). The use of PFGE to study bacterial strains is of great significance in tracing the source of infection and investigating the route of transmission in epidemiology.
Principle of PFGE
PFGE changes the swimming direction of the DNA molecules embedded in the agarose gel through the continuous change of the electric field. Small DNA molecules change faster than large ones, so that the gel shows an electrophoretic band pattern according to the size of the chromosome. In bacterial typing, the bacterial chromosomal DNA is embedded in a gel block, hydrolyzed with protease, and restriction endonucleases with rare enzyme cutting sites are used to digest the bacterial genomic DNA. The obtained DNA fragments are separated in an agarose gel applied with a pulsed electric field to produce different DNA bands. The density of DNA bands reflects the content of DNA in bacteria and the size of DNA molecules to a certain extent, and finally achieves the purpose of strain typing.
Our PFGE Service May Help You in The Following Areas (including but not limited to the following):
- Study the genetic differences between strains
- Look for possible connections in the cases
- Correlation analysis of strains isolated from living environment and strains isolated from patients
- Assist in tracking the source of infection
Workflow of PFGE Service at Creative BioMart Microbe
* You only need to provide us with a sample of the strain and mark the sample (please transport the sample under low temperature conditions). We will send you the test data and test report in time.
Pulse time, pulse angle, buffer and endonuclease are all factors that will affect the experimental results. Our technical experts have a wealth of experimental experience and can optimize these parameters as much as possible to present better experimental results to customers. At the same time, Creative BioMart Microbe has established a strict and complete quality management system to ensure that we provide customers with accurate and reliable experimental results.
If you want to know more about this service, please feel free to contact us by phone or email, or you can consult through our inquiry form. We will ensure to provide you with efficient and high-quality experimental outsourcing services.
