Bacteriocin Antibacterial Activity Determination
Bacteriocin is a kind of bacteriostatic product produced by some bacteria, which can inhibit a variety of gram-borne bacteria. We provide a variety of bacteriocin antimicrobial activity assay methods, hoping to provide effective help and guidance for the field of biotechnology.
Including land source bacteriocin is bacteriocin research subject, there are nearly thousand land source bacteriocins have been found and streptococcus element (Nisin) is the most thorough research bacteriocin, because of it has recognized the GRAS (Generally Regarded as Safe) properties.
Table 1. The classification of bacteriocins
|
Classification |
Feature |
Example |
Strains |
| I |
It contains modified amino acids and is stable to heat, including elongated cationic peptides, spheric peptides, lasso peptides, etc |
Nisin A |
Lactococcus lactis |
| Lacticin 3147 |
L. lactis |
| Cinnamycin |
Streptomyces cinnamoneus |
| Mersacidin |
Bacillus sp. |
| Sviceucin |
S. sviceus |
| II-a |
It contains amino end YGNGV conservative sequence, has inhibitory effect on listeria |
Pediocin CP2 |
Pediococcus acidilactici |
| II-b |
The two sequences work together |
Thuricin CD |
B. thuringiensis |
| II-c |
N-C terminal covalently bond to form a conserved cyclic peptide backbone |
Gassericin A |
L. gasseri |
| II-d |
Linear, not fully modified, non - staphylococcin similar to bacteriocins |
Bactofencin A |
L. salivarius |
| III |
High molecular weight and thermal instability |
Colicin V |
E. coli |
The bacteriocins produced by different bacteria have different antibacterial activities. Generally speaking, bacterin produced by G+ has antibacterial activity only to other G+. There are also some special bacterins that have antibacterial activity not only to G+ but also to some G−, such as Nisin A and mutacin-ny266. The Creative BioMart Microbe Laboratory provides a variety of suitable testing methods for testing the antibacterial activity of the tested microorganisms and has a set of standard operating methods that meet the requirements for the determination of antimicrobial activity of world-class microbial source enzymes.
We used Agar block method, Whatman filter paper method and other methods to determine the antibacterial activity of the samples to be tested. The principle of these methods is to determine the antibacterial activity of the samples to be tested by testing the size and diameter of the forming antibacterial ring.
Figure 1. The antibacterial activity of two kinds of tested bacteria was measured (Schematic diagram)
Criteria for determination of antimicrobial activity (part):
- ASTM E2149(Quantitative) Standard Test Method for determining antimicrobial activity of antimicrobial agents under dynamic contact conditions.
- GB 4789.43 for the determination of antibacterial activity of food microbial source enzyme preparations.
- YY/T 1477.1-2016 for the determination of antibacterial activity of contact wound dressings.
Why Choose Creative BioMart Microbe?
- Have a variety of bacteriocin assay methods, including AGAR diffusion, turbidimetric, radiation, ELISA kits, etc.
- The influence of organic acids and hydrogen peroxide was excluded when bacteriocin antimicrobial activity was tested.
- Has short and quality testing period, low cost, complete experimental scheme, and scientific, fair and accurate testing report issued.
- The accuracy of the experiment is high.
- With the international advanced testing equipment, has a special laboratory for scientific research.
- All experiments sign confidentiality agreements and pay attention to the protection of customer privacy.
- Ensure 24/7 online service.
Workflow of Bacteriocin Antibacterial Activity Determination at Creative BioMart Microbe
Our laboratory keeps a close eye on the development of science and technology and uses the latest test equipment and methods. It also provides other measurement, testing and analysis services as well as antimicrobial activity assays. Creative BioMart Microbe is your best partner. If you are interested in our agricultural pathogen testing services, please feel free to contact us. Creative BioMart Microbe looks forward to cooperating with you.
Reference
- Ramu, R.; et al. Bacteriocins and their applications in food preservation. Critical Reviews in Food Science and Nutrition. 2015, DOI: 10.1080/10408398.2015.1020918.
