Antimicrobial Susceptibility Testing in Biofilm Mode

Summary of the Antimicrobial Susceptibility Testing in Biofilm Mode

Biofilm is composed of organized bacterial communities, attached to living or abiotic surfaces. Persistent infection is a problem that plagues clinical microbiologists and clinicians, and the formation of biofilm is considered to be one of the most relevant driving factors for persistent infection. Compared with the planktonic bacteria involved in the acute processes, the resistance of biofilms to antimicrobial agents is significantly increased. A large amount of data shows that biofilms can tolerate antibiotics and disinfectants that are 100-1000 times higher than that of planktonic cells.

Therefore, there is a lack of correlation between the results of conventional susceptibility tests using planktonically growing bacteria with biofilm-growing bacteria associated with chronic infections. So, scientists are increasingly interested in antimicrobial susceptibility testing in biofilms. They have developed a variety of biofilm antimicrobial susceptibility testing methods, and used the pharmacodynamic parameters obtained in these studies to discuss their applications in clinical microbiology, and provide scientific guidance for treatment strategies.

The Antimicrobial Susceptibility Testing in Biofilm Mode Provided by Creative BioMart Microbe

Creative BioMart Microbe has accumulated a lot of experience in biofilm research. Our professional microbial team and advanced technology platform can help customers solve all the challenges encountered in the research process. Regarding antimicrobial susceptibility testing in biofilm mode, we provide closed systems (batch culture) and open systems (continuous culture). The closed models have better high-throughput characteristics and simpler operations, while the open models can better control the growth parameters and dynamics.

Closed Systems

Microtitre plate method: The operation is simple and rapid. One of the major disadvantages is that the absence of a relationship between biomass and biofilm viability.
Calgary biofilm device: It can rapidly and reproducibly determine the biofilm susceptibilities to antibiotics.

Open Systems

Flow cell: Simulates biofilm formation, enabling real-time non-destructive confocal laser scanning microscope (CLSM) analysis.
CDC Biofilm Reactor: Allows the study of seeding planktonic cells by sampling the bulk fluid phase.

Figure 1. Antimicrobial susceptibility testing with the flow cell biofilm model (Macià, M.D.; et al. 2014)

As an outstanding microbiological research institution, Creative BioMart Microbe provides high-quality antimicrobial susceptibility testing in biofilm mode to help customers research biofilms more efficiently. Our excellent technology platform can realize a variety of biofilm technical services, and solve all the difficulties encountered in research for customers. The praise from customers all over the world is the proof of our quality service.

If you want to know more about this service, please feel free to contact us by phone or email, or you can consult through our inquiry form. We will ensure to provide you with efficient and high-quality experimental outsourcing services.

Reference

Macià, M.D.; et al. Antimicrobial susceptibility testing in biofilm growing bacteria. Clinical Microbiology and Infection. 2014, 20: 981-990.