Disinfectant Testing for Pre-Saturated Towelette

Summary of the Disinfectant Testing for Pre-Saturated Towelette

In general, the disinfectant testing for pre-saturated towelette is a modified version of the AOAC germicidal spray products test. Unlike the germicidal spray products test, this method is to wipe the contaminated test surface with a disinfectant towelette instead of spraying it with a disinfectant.

Experimental Steps of the Disinfectant Testing for Pre-Saturated Towelette Provided by Creative BioMart Microbe

Dry the culture (48 hours) of an individual species of bacteria onto a number of clean, sterile microscope slides.

Place the dry and contaminated microscope slides in Petri dishes and wipe them with pre-saturated towelettes separately. According to the purpose of the test, 10 or 60 contaminated test surfaces can be treated with pre-saturated disinfection wipes. 

After wiping the test surfaces with towelettes, let them rest in the Petri dish for the specified contact time.

After the contact time has elapsed, transfer the treated test surface to a sterile container containing liquid growth medium for 48 hours at intervals of time. The growth medium can immediately neutralize the disinfection effect that exists on the pre-saturated towelette.

After incubation, record the number of tubes with growth of target microorganisms. At least 59 of the 60 surfaces wiped with the towelettes must prove to be fully disinfected (no growth of target microorganisms detected in the test tubes containing the neutralizing growth medium) to pass the test.

To "pass" a 10 carrier test, complete disinfection must take place on all of the wiped test surfaces.

Advantages of the Disinfectant Testing for Pre-Saturated Towelette

• This method is approved by the EPA for data submission in conjunction with disinfectant towelettes sold in the USA.
• This method is a "high level" test for germicidal wipes. The test products must have significant biocidal (germ-killing) activity within a relatively short (less than 10 minutes) time frame to "pass" the test.

Disadvantages of the Disinfectant Testing for Pre-Saturated Towelette

• Due to the ambiguity of the official method, the EPA pre-saturated germicidal towelette test method is subject to additional variability. (For example, the humidity level during the drying process of the glass test surfaces and the concentration of bacteria on the glass test surfaces are difficult to standardize.)
• The EPA pre-saturated germicidal towelette test method is fundamentally similar to the AOAC germicidal spray products test, and is variable on the basis of statistics alone.
• Because the pressure and technique of using pre-saturated towelettes to treat surfaces varies from different laboratories, the variability of this experiment is increased.

Creative BioMart Microbe is a professional biotechnology company that provides various analysis and testing services related to microorganisms and disinfectants. We have advanced technology platforms, experienced technical teams, and a complete quality management system. Individualized solutions can also be customized according to the different needs of customers.

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