Pichia pastoris Protein Expression System
Summary of the Pichia pastoris Protein Expression System
The yeast expression system is a powerful tool for studying eukaryotic proteins, including Pichia pastoris expression system and Saccharomyces cerevisiae expression system. They have the function of post-transcriptional modification and are suitable for the stable expression of functional foreign proteins. At the same time, they have the ability to sterically fold and glycosylate proteins, which greatly improves the solubility of proteins. Compared with insect and mammalian expression systems, Yeast expression system has the advantages of low cost, simple operation, short cycle, and large-scale fermentation. It is an ideal recombinant eukaryotic protein production tool.
Pichia pastoris can also be used as a substitute for Escherichia coli, because as yeast, Pichia pastoris not only has many advantages of advanced eukaryotic expression systems, such as protein processing, folding and post-translational modification, but also is as easy to operate as E. coli. At present, Pichia pastoris has been used to successfully produce various functional proteins, including enzymes, engineered antibody fragments, antigens, and engineered protein scaffolds. During the experiment of yeast protein expression, there are many factors that will affect the experimental results, including induction time and temperature, copy number of the target gene, culture conditions, purification methods, etc., which will cause differences in experimental results.
The Pichia pastoris Protein Expression System Provided by Creative BioMart Microbe
The expression and purification of eukaryotic proteins is a process that requires many attempts, and the negligence of any step may cause the efforts for months to be wasted. Creative BioMart Microbe has rich experience in this field, and can maximize the expression potential of Pichia pastoris, improve the expression success rate and expression level, and optimize the induction conditions and purification strategies according to the characteristics of the target protein. In addition, our Pichia pastoris expression platform is a simple and low-cost system. The length of the oligosaccharide chain of the expressed protein (average of 8-14 mannose residues per branch) is very close to that of higher eukaryotes, and the probability of activity is much higher than that of the prokaryotic system.
Advantages of Our Service
- Able to produce complex proteins
- High success rate
- High cell density/high protein yield
- Process controllable
- The proteins produced are not contaminated with endotoxins or viruses
- Can carry out small-scale and commercial-scale fermentation production
- Cost-effective
Creative BioMart Microbe is a biotechnology company with many years of experience in protein expression and purification. The advanced technology platform we have established is jointly managed by professional biochemical experts and protein experts. Our professional knowledge and perfect after-sales service have made us a trusted service provider for many customers.
If you want to know more about this service, please feel free to contact us by phone or email, or you can consult through our inquiry form. We will ensure to provide you with efficient and high-quality experimental outsourcing services.
