Quantitative Analysis of Microbiome
Microbial community research has a key position in different fields such as agriculture, medicine, environment, ecology and industry. In addition to the analysis of the microbial species composition in the sample, the microbiome research also requires the accurate quantification of certain bacteria. Creative BioMart Microbe provides customers with a variety of solutions for absolute quantification of the microorganisms, helping customers conduct in-depth research on microbial communities.
Quantification of Microbiome Samples
With the development of high-throughput sequencing technology, the analysis and research of microbial communities have been advanced. The application of 16S rRNA technology can obtain information on the species composition of the microbial community sample and the relative abundance of a certain bacteria. Although relative quantitative analysis plays an important role in promoting the study of microbial communities, relative abundance cannot reliably reflect the actual abundance of microorganisms. Relative quantitative analysis ig nores the differences in overall microbial biomass between different samples, which may lead to deviations in the analysis results. Relative quantitative analysis is not conducive to the in-depth study of the function of the microbial community, so it is necessary to conduct an absolute quantitative analysis of the microbial community. Absolute quantitative analysis can better reflect the true changes of the sample bacterial community, and is a better tool for microbial community research than relative quantitative analysis.
Solutions for Absolute Quantitative Analysis of the Microbiome
- 16S rRNA gene amplicon sequencing & Quantitative PCR
Quantitative PCR (qPCR) is widely used for the absolute quantification of bacterial content, and it is the gold standard for quantifying the absolute abundance of microorganisms. By combining the conventional 16S rRNA gene amplicon sequencing with qPCR, the abundance of taxon in microbial community samples can be absolutely quantified. The total copy number of the sample can be obtained by qPCR, and then the relative abundance of a single taxon can be obtained by using 16S rRNA gene amplicon sequencing, and the absolute abundance of a taxon can be inferred by combining the two data.
- 16S rRNA gene amplicon sequencing & digital PCR
Digital PCR (dPCR) overcomes the limitations of qPCR and can perform absolute quantification of DNA without a standard curve, reducing the time and cost of research. dPCR is a high-throughput absolute quantification method that can achieve precise quantification of nucleic acids and has the advantage of high sensitivity.
- 16S rRNA gene amplicon sequencing & flow cytometry
Flow cytometry can quickly and reliably detect microorganisms, including microorganisms that cannot be cultured. Flow cytometry can also distinguish and quantify live and dead bacteria in the microbial community. Using flow cytometry to obtain the total number of cells in the sample, and then through 16S rRNA gene amplicon sequencing to obtain the relative abundance of a taxon, combining the two data can infer the absolute abundance of some taxon.
Why Choose Creative BioMart Microbe?
- Quantitative technology with high sensitivity and specificity
- Optimized primer selection to reduce deviation and improve the accuracy of results
- One-stop service starting from DNA extraction, customized solutions based on different sample types
- Quality control of multiple links to reduce the accuracy reduction or underestimation of abundance that may be caused by each link
Workflow of Quantitative Analysis Service for Microbiome at Creative BioMart Microbe
Creative BioMart Microbe's microbial analysis platform will greatly promote your research projects. If you want to know more about this service, please feel free to contact us< by phone or email, or you can consult through our inquiry form<.
