Metabolite Identification Service

Metabolite identification is one of the important contents of metabolomics research, and it also plays an important role in the field of drug development. Creative BioMart Microbe has multiple technical platforms that can be used for metabolite identification and structural analysis, and can provide customers with fast and accurate metabolite identification services. We can also design a personalized analysis plan according to your specific research situation and research purpose.

The identification of metabolites includes putative identification and definitive identification. Putative identification is identification using one or two molecular properties. Definitive identification is the use of two molecular properties compared to an authentic chemical standard. Therefore, definitive identification is more accurate than putative identification, and it is generally performed after putative identification. Metabolite identification plays a key role in metabolomics research. Nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS) are the main tools of metabolomics and important analytical methods for metabolite identification, analysis, and structural elucidation. MS-based analytical methods, such as liquid chromatography–mass spectrometry (LC–MS), gas chromatography–mass spectrometry (GC-MS), etc., can cover many classes of secondary metabolites including amino acids, sugars, lipids, and organic acids.

Metabolite Identification Service

The identification of metabolites is a difficult point in metabolomics researches. Creative BioMart Microbe provides metabolite identification and analysis services through multiple technical platforms to help customers accelerate metabolite-related researches.

LC-MS: Combine the high separation ability of chromatography with the high sensitivity and selectivity of mass spectrometry, it is often used for the analysis of water-soluble small molecules and lipid macromolecules, such as amino acids, sugars, alcohols, organic acids, amines, TCA cycle intermediates, etc.

Liquid chromatography–high resolution mass spectrometry (LC–HRMS): HRMS has the characteristics of high mass precision, high sensitivity, high scanning speed, and high stability.

LC-MS/MS: Using MS/MS to perform neutral loss scanning, precursor ion scanning, and product ion scanning respectively, metabolites can be quickly found and their structures identified.

GC-MS: Combines the advantages of high separation efficiency and accurate quantification of GC with the advantages of high sensitivity, strong identification ability and fast response of MS, but involves hydrolysis or derivatization of samples. Commonly used for targeted analysis of water-soluble metabolites, some lipids and organic acids.

NMR Spectroscopy: It can provide a large amount of molecular structure information, but has high requirements for samples. It is generally used for the identification and analysis of simple samples or purified samples.

Why Choose Creative BioMart Microbe?

• Comprehensive metabolite identification technology platform: MS, LC-MS, LC–HRMS, LC-MS/MS, GC-MS, NMR.
• The one-stop service from sample processing to the issuance of identification and analysis reports realizes the rapid connection between upstream and downstream research.
• Experienced technical team and expert team ensure to provide customers with accurate and reliable experimental data and analysis reports.
• Personalized solutions to meet the research needs of different customers.
• Timely feedback and communication to ensure the accurate execution and repositioning of the test plan.
• Competitive prices help control research costs.

Workflow of Metabolite Identification Service

Creative BioMart Microbe provides comprehensive microbial metabolite analysis solutions. If you want to know more about metabolite identification services, please feel free to contact us. We also provide metabolite extraction, content determination, and metabolomics analysis services.

References:

1. Shuikan, A. M.; (2020) Enhancement and Identification of Microbial Secondary Metabolites. Najjari, A.; Cherif, A.; Sghaier H. & Ouzari, H. I. (Eds.) Extremophilic Microbes and Metabolites.
2. Xiao, J. F., Zhou, B. & Ressom, H. W. (2012). Metabolite identification and quantitation in LC-MS/MS-based metabolomics. TrAC Trends in Analytical Chemistry, 32(1), 1-14.