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Genetic Method Testing Service

Antimicrobial susceptibility testing (AST) is the measurement of the susceptibility of bacteria to antibiotics. It is used because bacteria may have resistance to some antibiotics. Considerable advances have been made in the AST which can be divided into two categories: phenotypic methods and genetic methods.

With the continuous progress of the world's biomedical industry and the gradual update of detection technology, genetic method or nucleic acid methods shines with its advantages such as accurate quantitative analysis and short operating procedures comparing the qualitative ADD method and the semiquantitative MIC method.

Schematic overview of genetic testing methodsFigure 1. Schematic overview of genetic testing methods

Creative BioMart Microbe provides many kinds of genetic methods assay services to detect whether bacteria possess genes which confer antibiotic resistance (including but not limited to):

  • Polymerase chain reaction (PCR)
  • Real-time quantitative PCR(RT-qPCR)
  • DNA microarray
  • DNA chips
  • RNA markers
  • Loop-mediated isothermal amplification (LAMP)

The genetic tests provided by Creative BioMart Microbe incorporate nucleic acid techniques similar to those used for organism identification but modified to detect known resistance genes or mutations, such as the imipenem, colistin, ciprofloxacin and the most classic gene, mecA, a gene for oxacillin resistance in S. Aureus, which is considered resistant to most beta-lactam drugs regardless of apparent susceptibility results. In addition, Creative BioMart Microbe also provides a variety of methods including electrophoresis, southern blotting, and other DNA sequencing analysis methods to demonstrate the presence of the genes.

The Advantages of Genetic Method Testing Service Provided by Creative BioMart Microbe:

  • We have high quality control (QC) testing to ensure the accuracy of test results, which guidelines are available from the Clinical and Laboratory Standards Institute (CLSI)
  • Hundreds of bacterial strains, including ESKAPE pathogens (S. aureus, K. pneumoniae, A. baumanii, P. aeruginosa, Enterobacter sp, etc.), multi drug-resistant clinical isolates, and multiple fungal strains, are available in our inventory for immediate testing.
  • Creative BioMart Microbe has a complete and comprehensive antimicrobial susceptibility testing technology system and a complete laboratory testing technology platform in the industry, which can provide personalized testing services.
  • The experienced technical team always ensures the implementation of strict and standardized experimental procedures to ensure the reliability and repeatability of the results.
  • Short service cycle and competitive price.
  • Urgent services are available.
  • Ensure 24/7 online service.

Workflow of Genetic Method Testing Service in Creative BioMart Microbe

Genetic Method Testing Service

Our ADD testing service, MIC testing service and genetic testing service about AST service can help clinical or non-clinical customers to test the antimicrobial susceptibility and effectiveness of various types of gram-bacteria and fungi.

The expert team of Creative BioMart Microbe has always been able to provide our customers with efficient and accurate AST testing services and has been a trusted partner of our customers.

If you are interested in our genetic testing service or AST testing services, please feel free to contact us. Creative BioMart Microbe looks forward to cooperating with you.

References

  1. Belkum, A. V.; et al. Innovative and rapid antimicrobial susceptibility testing systems. Nature Reviews Microbiology. 2020, 18(5): 299-311.
  2. Pulido, M.R.; et al. Progress on the development of rapid methods for antimicrobial susceptibility testing. Journal of Antimicrobial Chemotherapy. 2013, 68(12): 2710-2717.
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