Quantitative Analysis of Bacteria in Biofilm

Summary of the Quantitative Analysis of Bacteria in Biofilm

Biofilm can be regarded as a complex microbial community that grows at the interface, can interact closely with the surrounding environment, and has characteristics that help organisms survive under harsh conditions. Unfortunately, biofilms can cause many medical problems. For example, it can cause 60-80% of microbial infections, bring challenges to the diagnosis and treatment of diseases, and contaminate equipment and cause societal and industrial concern.

Although the research on the complex characteristics of biofilms has made great progress in the past few decades, there is still much work to be done. Whether in planktonic or biofilm cultures, one of the most basic and common types of bacteria measurements is to determine how much is present. Multidisciplinary methods have been used to solve these problems, not only to study the traditional biological properties of biofilms, but also to study their physical, dynamic chemical, and material properties. Currently, a variety of direct and indirect methods have been used to quantify cells in biofilms.

Figure 1. Light microscopy of biofilms (Wilson, C.; et al. 2017)

The Quantitative Analysis of Bacteria in Biofilm Provided by Creative BioMart Microbe

Creative BioMart Microbe has introduced many microbiologists and built an advanced technology platform to provide a variety of direct and indirect measurement methods for the quantitative analysis of biofilms. It should be noted that these methods all involve the use of a commercially available homogenizer and vortex mixing to homogenize the biofilm to disperse the cells in a liquid medium.

The direct quantification methods we provide include plate counts, Coulter cell counting, microscopic cell counts, flow cytometry and fluorescence microscopy. In addition, DNA, RNA, and polysaccharide quantification are also provided for analysis, but it is recommended to combine these methods with more direct methods.
The indirect quantitative methods we provide include determination of dry mass, microtiter plate assay, total organic carbon, ATP bioluminescence, fluorescence spectroscopy, total protein, and quartz crystal microbalance.

At present, biofilm research has received more and more attention, and Creative BioMart Microbe will focus this work on interdisciplinary collaboration. In addition, our researchers have professional knowledge to ensure high-quality services while planning interdisciplinary cooperation.

If you want to know more about this service, please feel free to contact us by phone or email, or you can consult through our inquiry form. We will ensure to provide you with efficient and high-quality experimental outsourcing services.

Reference

Wilson, C.; et al. Quantitative and Qualitative Assessment Methods for Biofilm Growth: A Mini-review. Res Rev J Eng Technol. 2017.