Bacterial Absolute Quantitative Sequencing
The 16S rRNA amplicon sequencing technology can objectively restore the structure and relative abundance of the microbial community, and realize the composition analysis of the microbial community. However, the conventional 16S rRNA amplicon sequencing technology obtains the relative abundance information of a certain group of microorganisms, and cannot reflect the change in its absolute abundance. Creative BioMart Microbe combines 16S rRNA amplicon sequencing technology and quantitative PCR (qPCR) technology to establish a unique bacterial absolute quantitative sequencing platform. Through our bacterial absolute quantitative sequencing platform, customers can obtain the absolute copy number of each species in the microbial community.
Bacterial Absolute Quantitative Sequencing Platform
qPCR technology can be used for absolute quantification of bacterial content and is the gold standard for absolute quantification of the abundance of microorganisms. However, the use of qPCR for quantitative analysis of bacteria has the disadvantage of unstable experimental results. In addition, qPCR experiments involve primer design and have higher requirements for primer specificity. The microbial community has the characteristics of diverse species, and this will increase the difficulty of using qPCR technology to analyze the microbial community. Another challenge for the analysis of bacteria in environmental samples by qPCR is that the samples contain humic acid, which can affect the accuracy of qPCR results.
Creative BioMart Microbe combines 16S rRNA amplicon sequencing technology and qPCR technology, which can not only realize the routine analysis of bacteria, such as community composition analysis, alpha diversity analysis, and beta diversity analysis, but also calculate the absolute copy number of 16S rRNA genes of specific species in the sample. The bacterial absolute quantitative sequencing platform makes up for the shortcomings of PCR quantitative experiments and realizes high-throughput absolute quantitative analysis of bacteria in samples from soil and water.
Creative BioMart Microbe's Bacterial Absolute Quantitative Sequencing Platform Can Help Customers in The Following Aspects:
- Alpha diversity analysis
- Beta diversity analysis
- Analysis of bacterial community composition
- Analysis of the total copy number of bacteria
- Analysis of the absolute copy number of species
Sequencing Scheme
- The recommended regions for absolute quantitative bacterial sequencing are V3, V4, V3+V4, V4+V5
- ≥150,000 effective sequence per sample
- Raw data Q30 more than 70%, Clean data 30 more than 80%
Technical Advantages of Absolute Quantitative Sequencing
- High throughput, more than 150000 reads per sample
- Realize the detection of trace bacteria in the microbial community
- Simultaneously carry out relative quantitative analysis and absolute quantitative analysis. Realize joint comparative analysis of microbial communities
- Exclude the influence of PCR inhibitors on quantitative results
Sample Requirements
Pre-extracted DNA (in order to ensure the accuracy and reliability of the analysis results, customers need to ensure that the DNA samples provided meet the requirements, or directly perform DNA extraction by our technicians): The total sample volume is required to be > 25 μL, and the genomic DNA concentration ≥ 20 ng/μL, OD260/280=1.7-2.0
Why Choose Creative BioMart Microbe
- Unique quantitative sequencing platform
- Accurate, reliable and efficient bacterial quantitative sequencing analysis
- Provide professional and authoritative analysis reports
- One-stop service and reasonable price
Workflow of Bacterial Absolute Quantitative Sequencing Service
The bacterial quantitative sequencing platform established by Creative BioMart Microbe makes up for the shortcomings of conventional 16S rRNA amplicon sequencing technology and brings new possibilities for microbiology research. If you want to know more about our bacterial quantitative sequencing technology, please feel free to contact us. You can also choose to conduct service-related consultations through our inquiry form, and our staff will reply to you as soon as possible.
