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HFA Mice Model Construction

In recent years, important progress has been made in the study of intestinal flora in the signal transduction pathway of fat metabolism and inflammation pathway. At present, a variety of human flora associated (HFA) animal models have been reported, which is conducive to the direct study of the relationship between intestinal flora of patients and diseases (especially obesity).

The establishment of non-bacterialized animal models (such as HFA animal models) carrying human flora has provided a better model for studying the ecosystem and metabolism of human intestinal flora. Creative BioMart Microbe can provide HFA mice model for the majority of hospitals and research institutions.

Introduction to the HFA Mice Model

Through the fecal flora transplantation method, the human intestinal flora is "placed" on the mouse model to prepare the humanized mouse model, and the evaluation can be carried out, which can simulate some human diseases.

Process of HFA Mice Model Construction

HFA Mice Model Construction

  • A certain number of sterile mice, half male and half female, were independently bred by the laboratory animal center of the company. All sterile mice were cultured according to the national standards for experimental animals, laboratory operating specifications and standard operating procedures.
  • Collect fresh feces from patients or other subjects and dilute them into fecal suspension by adding anaerobic broth or phosphate buffer solution at an appropriate proportion (our method is 10 times dilution) in the ultra-clean workbench.
  • Appropriate fecal suspension was poured into the stomachs of sterile mice in the experimental group, and HFA mice were obtained after 3 weeks of colonization.

HFA Mice Detection

  • Total DNA extraction from mouse feces.
  • The PCR - DGGE detection.
  • Similarity UPGMA cluster analysis and PCA analysis.
  • Statistical analysis.

Our scientists will provide suggestions on the construction of different HFA mouse models according to different real situations of customers. The HFA mouse model service provided by Creative BioMart Microbe has the following advantages:

  • The genetic background of the model was clear: the genetic background and source of the HFA mouse model were clear.
  • Intellectual property of the model is clear and guaranteed: The mouse model constructed by our company is based on extensive patents and licensing agreements to ensure clear intellectual property rights.

HFA mice partially simulated the microbiome of patients with a disease such as obesity, and mice of the same sex as the patient did better. The HFA mouse model established by our company provides a new choice for the further study of the relationship between diseases (such as obesity) and intestinal flora.

Creative BioMart Microbe is able to construct a HFA animal model by selecting sterile mice and inoculating the feces of obese patients, and denatured gradient gel electrophoresis (DGGE) was used to evaluate the colonization of intestinal flora of patients in sterile mice.

If you want to know more construction information and price information, please feel free to contact us and we will reply to you as soon as possible.

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