Bacterial Mutants Isolation Service

Bacterial mutants isolation is very important for bacterial genetics research. The frequency of naturally occurring mutations is very low, so the enrichment technology can be used to enrich a large group of wild-type bacteria. The bacteria that survived the enrichment test will be inoculated into the conditions that allow the growth of mutants to screen for mutants. With the progress of molecular biology and the rapid development of sequencing technology, people can be directed to obtain microbial mutants and master their genetic background. The transformed bacteria were obtained by artificial transformation, then the transformed bacteria were cloned and purified, and a random monoclone was selected and sequenced to understand the genetic characteristics of the strain.

Bacterial Mutants Isolation Service

Many microbial strains are difficult to culture, easy to cross contamination and other reasons, therefore, it is necessary to carry out active and effective strain isolation and purification and other genetic identification. The isolation and purification of bacterial mutant strains is a crucial step to study the mechanism of bacterial action. Creative BioMart Microbe provides several strategies to accelerate the process of bacterial mutant isolation.

• Preparation of Medium and Strain Culture

We can supply a variety of reagents required for the preparation of the medium or we can configure the agar plates required for different strains. At the same time, we have established a culture preservation and management platform, which can select the required strains for research.

• Isolation of Bacterial Mutant Strains

There are many methods for the isolation of bacterial mutant strains. The conventional methods include streaking on agar plate, coating on the surface, or mixing with agar medium and pouring on the plate to obtain a single colony.

• Purification of Bacterial Mutants

Bacterial mutants can be purified by conventional column chromatography, GDP-Sepharose affinity chromatography, and the results can be analyzed by SDS-polyacrylamide gel electrophoresis.

• Whole Genome Sequencing of Bacterial Mutants 

We have built an advanced high-throughput sequencing platform. First, we used phenol-chloroform extraction to extract and purify DNA. Then, the extracted genomes were sequenced for second and third generation, and the whole genome sequence was analyzed.

Why Choose Creative BioMart Microbe?

• Advanced molecular biology platform and strain conservation management platform to support your microbial genetics research
• Strict quality control to ensure efficient and accurate experimental results
• Customized service strategy to meet the personalized research needs of different customers
• Clear contract content, timely feedback and communication
• One-stop research service platform to stimulate the vitality of research and innovation

Workflow of Bacterial Mutants Isolation Service

Creative BioMart Microbe focuses on providing microbiology-related technical services for scientific research and industry, and the one-stop service realizes the rapid connection of upstream and downstream research. Please feel free to contact us for more details about our bacterial mutant isolation services.

Reference:

1. Jonak, J. Bacterial elongation factors EF-Tu, their mutants, chimeric forms, and domains: isolation and purification. J Chromatogr B Analyt Technol Biomed Life Sci. 2007, 849(1-2): 141-153.