Hard Surface Time-Kill Test for Disinfectants

Experimental Steps of Hard Surface Time-Kill Test for Disinfectants Provided by Creative BioMart Microbe

Prepare microbial cultures. For most bacteria, culturing in nutrient broth for 24 hours works well. For most fungi, a spore preparation from a saline wash works well.

Place a quantity of microbial culture (usually 0.010 ml to 0.020 ml) in the center of each of the series of sterile test surfaces. This inoculum can be spread on the sterile test surface in a circular pattern to achieve a thin and uniform coating with the test microorganisms.

To measure the initial microbial concentration, one or more untreated, inoculated test surfaces are harvested and microorganisms are counted.

The remaining inoculated test surfaces were treated with test products for different periods of time.

After the treatment, immediately place the test surface in a solution that neutralizes the disinfection action of the product, and cultivate and enumerate the microorganisms that survive the disinfectant or sanitizer treatment.

The results of the time-killing experiment are tabulated and reported, usually by charting the concentration of microorganisms on the test surface as a function of treatment time with the disinfectant or sanitizer.

Advantages of Hard Surface Time-Kill Test for Disinfectants

• The experimental model has strong practical significance.
• The experiment is relatively cheap and simple to operate.
• The experimental parameters are easy to control in the laboratory environment, so various disinfectants and sanitizer products tested can be effectively compared under the same conditions.

Disadvantages of Hard Surface Time-Kill Test for Disinfectants

• The characteristics of the test surface have an impact on the test results.
• Environmental parameters related to the drying of the microbial inoculum have an impact on the test results (for example, a low relative humidity will cause the microbial inoculum to dry faster than an inoculum dried at a high relative humidity).
• Some microorganisms are more tolerant to dryness than others, making it difficult to standardize the experiment.

Creative BioMart Microbe is a professional and advanced biotechnology company that provides a variety of disinfectant testing services for industrial disinfectants, household disinfectants, germicidal sprays, and disinfectant towelette, etc. We have accumulated a lot of experience in this field and have helped many customers around the world successfully solve their problems.

If you want to know more about this service, please feel free to contact us by phone or email, or you can consult through our inquiry form. We will ensure to provide you with efficient and high-quality experimental outsourcing services.