Analysis of the Interaction Between Microbial Biological Control Agents and Pathogens
Microbial biocontrol agents protect plants from the harmful effects of plant pathogens through various modes of action. It can control these plant pathogens and help suppress disease through direct antagonism, mixed interactions, or indirect antagonism. These interactions involve a variety of mechanisms such as parasitism, predation, antibiotic action, enzyme production, and competition for nutrients and space. Plant defense mechanisms induced by stimulus activation recognized by unique recognition receptors. With the continuous development of molecular biotechnology and the in-depth study of molecular biology in the field of microbiology, a comprehensive understanding of microbial biological control agents and their interactions at the cellular and molecular levels will help to screen effective and environmentally friendly biological agents, thus expanding the range of microbial biological control agents.
Figure 1. Functional groups of differentially expressed proteins in seedling hypocotyls from the soybean cultivar Williams 82 infected by P. sojae P6497 and P7076 (Jing M.; et al. 2015)
Analysis of the Interaction Between Microbial Biological Control Agents and Pathogens
In almost all cases, microbial biocontrol agents exhibit antagonism against plant pathogens, depending largely on the specific interactions that occur between the host, pathogen, and biocontrol agent. Creative BioMart Microbe provides several strategies for accelerating the analysis process of microbial biocontrol agent interactions with pathogens.
In-gel digestion is usually to cut the protein points in the glue into peptide segments by the protease (most commonly trypsin), and extract the peptide segments after enzyme digestion from the glue with solvent for mass spectrometry analysis.
We have established a protein histological analysis platform to analyze the proteome of microorganisms. For example, functional groups of differentially expressed proteins are studied.
- MALDI-TOFTOF MS Technology
The matrix-assisted laser desorption/Ionization time-of-flight Mass spectrometer (MALDI-TOFTOF MS) technology is mainly applied to the identification of bacteria, fungi and other microorganisms, including the identification of high abundance, stable expression and evolutionarily conserved ribosomal proteins in microorganisms.
- Strain Identification and Typing
Common methods for strain identification include: gene sequence homology analysis and PCR fingerprint-based identification, including restricted fragment length polymorphism (RFLP), random amplified polymorphic DNA analysis (RAPD), amplified fragment length polymorphism (AFLP) and single chain conformation polymorphism (SSCP), nucleic acid hybridization.
Why Choose Creative BioMart Microbe?
- The technical service team has many years of experimental experience and can identify strains from different sources.
- Our expert team will tailor a more suitable experimental solution to meet your downstream experimental requirements.
- Standardized molecular biological platform.
Workflow of Analysis Service for the Interaction Between Microbial Biological Control Agents and Pathogens
If you want to know more about this service, please feel free to contact us.
Reference:
- Jing, M., et al. Differential regulation of defense-related proteins in soybean during compatible and incompatible interactions between Phytophthora sojae and soybean by comparative proteomic analysis. Plant Cell Rep. 2015, 34(7): 1263-1280.
