Protein Expression System Evaluation

There are many common protein expression systems, including prokaryotic expression system, yeast expression system, insect cell expression system, mammalian expression system, etc. When you want to express a certain protein, which expression system should you choose? The protein expression system evaluation service provided by Creative BioMart Microbe is to help you solve this problem. We evaluate different protein expression systems to help you choose the one that is suitable for the protein you want to express.

Different Protein Expression Systems

Prokaryotic expression systems are mainly Escherichia coli expression system and Bacillus subtilis expression system. The Escherichia coli expression system has the advantages of high expression of target genes, low cost, simple culture conditions, rapid production, and simple transformation operations. It is suitable for expressing prokaryotic proteins and simple eukaryotic proteins. The Bacillus subtilis expression system has the advantages of no endotoxin, can secrete a large amount of certain extracellular proteins, simple culture conditions, rapid production, and not easy to form inclusion bodies, etc., and is suitable for the expression of extracellular proteins.

Yeast expression system has the advantages of simple culture conditions, rapid production, cost-effective, extensive post-translational modification, no endotoxin, and easy purification. It is a good choice for secreted proteins or intracellular expression, and is suitable for industrial strain improvement and amplification. Common yeast expression systems include Saccharomyces cerevisiae expression system and Pichia pastoris expression system.

Baculovirus-insect cell expression system has the advantages of large gene capacity, high expression efficiency of foreign genes, extensive post-translational modification, glycosylation similar to mammalian cells, and easy deglycosylation. It is an excellent expression system for proteins such as signaling proteins, cytokines, and kinases.

Mammalian expression systems are capable of instructing correct protein folding with sufficient post-translational modifications, including complex N- and O-glycosylation. Mammalian expression system products are close to natural protein molecules in terms of molecular structure, physicochemical properties and biological functions. Commonly used cell lines for mammalian expression systems include HEK293 and CHO, which are suitable for the expression of complex higher eukaryotic proteins.

Choosing from multiple protein expression systems requires consideration of several factors, including the size of the protein, the properties of the protein (whether post-translational modifications are required and whether disulfide bonds are present, etc.), the species of protein source (from prokaryotic or eukaryotic), the amount of protein required (whether the expression system is suitable for large-scale protein production), production cost, and the application of the protein.

Creative BioMart Microbe has many years of experience in protein expression, and can evaluate different protein expression systems according to customer needs, helping customers choose an appropriate expression system to meet research needs.

Why Choose Creative BioMart Microbe?

• With rich experience and professional technology, we can help customers solve various problems involved in protein expression.
• Different protein expression technology platforms provide customers with multiple choices.
• Efficient and reliable services help customers shorten the experimental cycle and increase protein production.
• Reduce protein production costs.
• Realize real-time technical communication and tracking services.

If you want to inquire about the details of protein expression assessment services, please feel free to contact us by phone, email or online form.