Custom Phage Display Solutions
BackgroundService ProcedureOur AdvantagesFAQs
Background
Phage display technology is a powerful tool for molecular discovery and engineering, widely employed in antibody development, peptide screening, receptor-ligand interaction studies, and epitope mapping. However, standardized workflows may not address the specific needs of complex or unconventional targets, unique selection environments, or highly specialized downstream applications.
Figure 1. Simplified antibody production process via phage display.
At Creative BioMart Microbe, we offer custom phage display solutions designed to overcome these challenges through tailored library construction, specialized biopanning strategies, and integrated post-screening analysis. Our flexible approach ensures that each project is strategically aligned with your scientific objectives, whether you are working in therapeutic development, diagnostics, synthetic biology, or functional proteomics. Contact us today to discuss your unique project!
Service Procedure
Service Details
Project Consultation and Feasibility Assessment
We initiate each project with an in-depth consultation to define the target type, application goals, screening format, and any known challenges. A feasibility assessment is provided to align expectations with achievable outcomes.
Custom Library Design and Construction (Optional)
- Targeted Antibody Libraries: Human, murine, camelid, or synthetic scFv/Fab/VHH formats
- Peptide Libraries: Linear or cyclic formats with tailored diversity and structure
- Specialized Modifications: Incorporation of unnatural amino acids, epitope tags, or chemical warheads
Bespoke Biopanning Strategy
- Target Presentation: Soluble proteins, membrane proteins, liposomes, viruses, live/fixed cells, or tissue sections
- Selection Conditions: Include negative selection, competitive elution, pH or redox-triggered elution, and cell-based selection
- Environmental Customization: Screening under physiological, hypoxic, acidic, or proteolytic conditions
Screening and Enrichment
High-throughput phage screening is conducted with real-time monitoring of enrichment efficiency. Additional analytical validation ensures selection fidelity.
Clone Analysis and Downstream Engineering
- Sanger or NGS-based sequence analysis
- Conversion to full-length IgG, Fc-fusion proteins, or custom formats
- Binding validation by ELISA, SPR/BLI, flow cytometry, or cell-based assays
Service Highlights
Library Types Offered
- scFv, Fab, VHH single-domain antibodies
- Linear & cyclic random peptide libraries (7–20mers)
- Targeted (focused) libraries using known scaffolds
- Mutagenesis libraries for affinity maturation
Biopanning Capabilities
- Solid-phase (plate, beads, membrane)
- Solution-phase (magnetic beads, in-solution targets)
- Cell-based (adherent or suspension cell lines)
- Tough targets: GPCRs, membrane proteins, PTM-specific antigens
Screening & Characterization
- Monoclonal and polyclonal phage ELISA
- DNA sequencing (Sanger or NGS-based)
- Binding kinetics via SPR or BLI (optional)
- Epitope binning & cross-reactivity testing
Our Advantages
- Fully Customizable Workflows: From library design to selection parameters, every aspect of the service is tailored to your scientific and technical specifications.
- Broad Target Compatibility: Expertise in handling membrane proteins, conformational epitopes, insoluble targets, and rare cell populations.
- Integrated Development Support: Smooth transition from binder discovery to functional expression, affinity maturation, and preclinical assay readiness.
- High-Diversity and Precision Libraries: Access to advanced display formats and proprietary construction techniques ensures robustness and reproducibility.
- Confidentiality and IP Assurance: Client IP and data are protected with strict confidentiality policies and optional project-based material transfer agreements (MTAs).
FAQs
Q: Can you work with targets that are difficult to purify or unstable?
A: Yes. We specialize in cell-based or membrane-associated selections, and offer alternative immobilization or display strategies for labile targets.
Q: What types of libraries can be constructed?
A: We offer a wide range of antibody (scFv, Fab, VHH) and peptide (linear, cyclic, chemically modified) libraries, all customizable in size, diversity, and format.
Q: Is it possible to incorporate unnatural amino acids or chemical groups in the library?
A: Yes. We support site-specific incorporation of non-canonical residues and functional warheads for specialized applications.
Q: How is the quality of the custom library ensured?
A: Library diversity, representation, and randomization fidelity are verified using next-generation sequencing (NGS) and statistical analysis.
Q: Can selected clones be directly developed into therapeutic candidates?
A: Yes. We offer downstream services for format conversion, expression, purification, and functional validation suitable for preclinical workflows.
References:
- Huang JX, Bishop-Hurley SL, Cooper MA. Development of anti-infectives using phage display: biological agents against bacteria, viruses, and parasites. Antimicrob Agents Chemother. 2012;56(9):4569-4582. doi:10.1128/AAC.00567-12
- Petrenko VA. Phage display's prospects for early diagnosis of prostate cancer. Viruses. 2024;16(2):277. doi:10.3390/v16020277
