The development of biotechnology provides more technical choices for the study of microorganisms. Creative BioMart Microbe has established a comprehensive gene expression analysis platform, covering many methods including real-time quantitative PCR, digital PCR, and DNA microarray, which can meet the research needs of most customers.

Gene Expression Analysis Platform

• Real-time quantitative PCR (qPCR)
  qPCR uses the fluorescent group (dye or probe) to the PCR reaction system, and uses the increase in the PCR process to monitor the PCR process. qPCR is a genetic expression analysis method with good repetitiveness, high accuracy and sensitivity, which is widely used in the gene expression research of microorganisms. We provide one -stop services from primer design and synthesis, RNA extraction, reverse transcription, qPCR to experimental data analysis.
• Digital PCR (dPCR)
  dPCR is a technique for absolute quantification of nucleic acid molecules. dPCR requires a limited dilution of PCR reaction material, followed by PCR amplification in different reaction chambers, and finally the starting copy number or concentration of the target molecule is obtained based on the Poisson distribution principle and the number and proportion of positive microdroplets. Unlike qPCR, which performs real-time fluorescence measurements for each cycle, the dPCR technique is used to collect the fluorescence signal from each reaction unit after the amplification is completed.
• DNA Microarrays
  DNA microarray technology uses the principle of base complementary pairing to measure nucleic acids qualitatively or quantitatively. Labeled cDNA from the sample is hybridized with probes of known sequences integrated on the microarray, and rapid detection of genetic information is achieved by detecting the hybridized probes at the corresponding positions. It has the advantages of rapidity, accuracy, high sensitivity as well as high throughput. Gene expression profiling of microorganisms can be achieved by DNA microarray technology.
• Northern Blotting
  Northern blotting is a technique based on molecular hybridization of nucleic acids to detect specific RNA. RNA samples are generally separated by agarose gel electrophoresis and transferred to a solid phase carrier. The hybridization reaction is then performed with a radioactive or non-radiolabeled probe and the hybridization signal is analyzed to complete a qualitative or quantitative analysis of RNA levels.
• Sequencing
  Creative BioMart Microbe provides sequencing technology services for gene expression profiling of microorganisms. RNA-Seq of microorganisms enables the quantification and annotation of microbial transcripts and the rapid localization of highly or lowly expressed genes. Compared to other gene expression analysis methods, sequencing technology has a higher throughput.

Why Choose Creative BioMart Microbe?

• One-stop service from sample processing to data analysis.
• Multiple technology platforms to meet the research needs of different customers.
• Short testing cycle and regular reporting of project progress.
• Real-time communication and resolution of your questions.
• Experienced technical team to ensure accurate and reliable analysis results.
• Provide detailed experimental records and professional analysis reports.

Workflow of Microbial Gene Expression Analysis Service

Creative BioMart Microbe has many years of experience in the field of microbiology and molecular biology and is able to customize appropriate gene expression analysis solutions for our clients. If you are interested in our gene expression analysis platform, please feel free to contact us for a more in-depth discussion.

• Real-Time Quantitative PCR