Bacteriocin Production & Parameters Optimization
Bacteriocin is an antimicrobial peptide synthesized by bacteria through ribosomes. It generally acts on the cell membrane of the target strain and can inhibit the growth of other bacteria with similar classification status by destroying the structural integrity of the membrane and causing leakage of cell contents, thus leading to bacterial death.
Bacteriocin can be derived from a wide variety of sources, such as gram-positive bacteria (G+), gram-negative bacteria (G−) or some ancient bacteria, such asEscherichia coli, Bacillus subtilis, Mutans streptococci, Streptococcus sanguis and lactic acid bacteria. There are nearly thousand land source bacteriocins have been found and streptococcus element (Nisin) is the most thorough research bacteriocin, because of it has recognized the GRAS (Generally Regarded as Safe) properties.
Creative BioMart Microbe can isolate a variety of bacteriocins and optimize medium composition (growth medium and glucose, nitrogen source and addition of Twenty20) and culture conditions (temperature, pH, stirring and inoculum volume).
Table 1. Complex media commonly used for cultivation of LAB and bacteriocin production (part)
| Culture media |
Producing strain |
Bacteriocin |
Remarks |
| Diluted whey, concentrated whey, MRS |
P. acidilactici NRRL B-5627 and L. lactis subsp. Lactis CECT539 |
Pediocin and nisin |
Nutrient sources were not adequate to increase production in diluted whey. Growth and productions in whey were significantly lower compared to MRS broth |
| MRS, M17 and BHI |
L. lactis subsp. Lactis ST34BR |
Bacteriocin ST34BR |
Production in MRS was higher compared to BHI and M17 |
| M17 and MRS |
P. acidilactici KKU 197 |
Bacteriocin KKU197 |
P. acidilactici KKU 197 displayed higher activity when grown in MRS compared to M17, although cell growth was similar |
| TGE and MRS |
P. acidilactici H |
Pediocin AcH |
TGE resulted in higher production levels compared to MRS broth |
| MRS and TGE |
P. acidilactici H |
Pediocin AcH |
Production in MRS was about 15% lower compared to TGE, although the final cell concentration was greater in MRS |
Bacteriocin growth usually follows a major metabolite growth-related kinetics that occurs during the exponential growth phase and stops once the stationary phase is reached. This means that the production of bacteriocins depends on the total biomass of the bacteria. Creative BioMart Microbe can not only isolate bacteriocins, but also improve the yield of antibiotics and optimize the titer of antibiotics through a series of production processes, which will realize a large amount of bacteriocins at a low cost for you.
Figure 1. Interaction of several fermentation factors during bacteriocin production.
Why Choose Creative BioMart Microbe?
- Depending on demand and after eliminating the effects of hydrogen peroxide and organic acids, we can isolate more than 300 strains at a time and screen the strains that produce bacteriocin.
- Provide multiple extraction methods for bacteriocin, including ammonium sulfate precipitation, organic solvent extraction, adsorption, filtration chromatography, etc.
- Realize the bacteriocin extraction method from crude to precise extraction, and the subsequent separation and purification is easier, with less impurities.
- Short and quality testing period, low cost, complete experimental scheme, and scientific, fair and accurate testing report issued.
- With the international advanced testing equipment, has a special laboratory for scientific research.
- All experiments sign confidentiality agreements and pay attention to the protection of customer privacy.
- Ensure 24/7 online service.
Workflow of Bacteriocin Production & Parameters Optimization at Creative BioMart Microbe
Our laboratory keeps a close eye on the development of science and technology and uses the latest test equipment and methods. We also provide other measurement, testing and analysis services as well as antimicrobial activity assays. Creative BioMart Microbe is your best partner. If you are interested in our testing services, please feel free to contact us. Creative BioMart Microbe looks forward to cooperating with you.
