Microorganisms are the most diverse living organisms that exist on earth. Microorganisms exist in almost all environments on earth, and they are an important component of ecosystems and are crucial to the global ecological cycle. Microorganisms generally exist in the form of microbial communities, and characterizing the structure, functions, and interactions of microbial communities is of key significance for related research on ecosystems, environmental restoration, and sustainable agriculture.
The microbial community has the characteristics of diversity. One gram of soil contains 2,000 to 8.3 million species, and most of the microorganisms have not been cultured, which further increases the difficulty of researches on the structure and function of the microbial community. At present, high-throughput technologies are used to characterize microbial communities, including sequencing technology and microarray technology.
GeoChip
GeoChip is a DNA microarray technology that independently designs specific oligonucleotide probes based on various functional genes related to microorganisms for high-throughput analysis of the diversity of functional genes in microbial communities. Compared with sequencing technology, GeoChip has higher repeatability and stability. GeoChip can not only reduce the interference of the host genome to the detection, but also ensure high reproducibility and high sensitivity of results between parallel samples. In addition, GeoChip has an automated experimental process and analysis platform, and the data is simple and can be used directly for statistical analysis. GeoChip 5.0 is the latest version of GeoChip technology, covering ~ 160,000 probes targeting ~ 370,000 encoded genes in ~ 1,500 gene families, and is more specific, sensitive and reproducible than other GeoChip technologies.
The GeoChip service provided by Creative BioMart Microbe can be used to analyze microbial communities from a variety of environments, including soils, aquatic ecosystems, extreme environments, contaminated habitats, and bioreactors.
Why Choose Creative BioMart Microbe?
- High specificity, sensitivity and reproducibility
- Specific probes based on functional gene design reduce the influence of host genome on detection results
- Targets a large number of gene families for broad coverage of target microorganisms
- Enables rapid, high-throughput analysis of microbial communities
- Short project cycle and high cost-effectiveness
Sample Requirements
- DNA: The sample concentration ≥ 50ng/μL, and the total amount ≥ 3μg. OD260/280 is required to be 1.8-2.0, and OD260/230 ≥ 1.5. No obvious DNA degradation, no RNA, protein and other impurities pollution.
- RNA: The sample concentration≥ 65ng/μL, and the total amount ≥ 3.5μg. OD260/280 is required to be 1.8-2.0, 23S/16S ≥ 1.0, RIN ≥ 6.0. No contamination by impurities such as genome and protein.
Workflow of GeoChip Service in Creative BioMart Microbe
If you want to know more information about this service, please feel free to contact us by phone or email, or you can consult through our inquiry form. We will ensure to provide you with efficient and high-quality experimental outsourcing services.
