Summary of the Recombinant Protein Expression and Purification

The use of bacterial systems for the expression of recombinant proteins has become a mature technology, but how to purify active proteins is still challenging. The purification of recombinant proteins is an important technology in biological research, because in order to study the function and structure of proteins in-depth, researchers need to isolate and purify these recombinant proteins from organisms. In this process, the proteinaceous substances can be removed according to the similarity between the proteins, and then the target recombinant protein can be separated according to the differences between the proteins.

At present, protein tags have been developed into efficient and convenient tools that can be used to simplify the protein purification process, improve the solubility of recombinant proteins, and allow easy tracking of proteins during protein expression and purification. In addition, visual protein tags enable proteins in living cells to be directly tracked through a microscope, which has become a useful tool for studying protein expression processes. In addition, proteins are affected by various factors in the expression process, such as temperature, time, pH, etc., so the control and optimization of these parameters is an indispensable step in protein expression and purification.

Recombinant Protein Expression and Purification Provided by Creative BioMart Microbe

Creative BioMart Microbe is a biotechnology company specializing in protein expression and purification. Our protein production services in bacterial systems include more than 30 protein expression tests and high-throughput parameter tests to ensure that the protein expression process is in optimal conditions. So that even proteins that are difficult to express have a high production probability.

Our service includes various tests:

• Recombinant plasmid construction  
• Protein sequence optimization
• Tag screen
• Optimization of expression conditions
• Purification method test
• Tag cleavage condition test
• Refolding method and formulation test.

The solutions and technologies provided by Creative BioMart Microbe have helped many researchers around the world solve the difficulties and challenges in protein expression and purification. Up to now, we have successfully expressed more than 1,000 target proteins. The vast amount of experience we have accumulated has made us a trustworthy protein expression and purification service provider.

Highlights

• Comprehensive condition screening
• Quality assurance
• Experienced technicians
• Powerful technology platform and data analysis tools
• One-stop service
• Cost-effective

If you want to know more about this service, please feel free to contact us by phone or email, or you can consult through our inquiry form. We will ensure to provide you with efficient and high-quality experimental outsourcing services.