Strain Isolation & Culture Service

Several biotechnologies have facilitated the study of microbial communities, such as high-throughput sequencing, mass spectrometry, and microarrays. The application of these techniques can only obtain relevant information such as the type and relative abundance of microorganisms in the sample, but not the specific strains. Strain isolation is to use the characteristics of strains to separate and screen specific strains from samples mixed with various microorganisms.

Creative BioMart Microbe focuses on the research and application in the field of microbiology. We not only have a strain identification platform, an omics analysis platform, but also an aerobic/anaerobic bacteria isolation and cultivation platform for microbial communities. Based on this technology platform, we have the ability to provide customers with efficient and reliable aerobic/anaerobic microorganism isolation and cultivation services. Our strain isolation and culture service can help customers obtain specific bacteria or fungi from many different types of samples.

Strain Isolation & Culture Service

The collected fresh samples need to be put into anaerobic or aerobic preservation solution before sending the samples (we can provide you with anaerobic or aerobic preservation solution). Samples can be freeze-dried powder of bacterial strains, bacterial liquid, bacterial strains grown on petri dishes, human or animal feces, intestinal tissue, soil, sludge, sewage, plant diseased strains or other samples.

• Before isolation and culture, high-throughput sequencing is required to detect the type and relative abundance of microorganisms in the sample, and to determine whether there is a target strain of interest to the customer.
• Determine the culture conditions and medium type according to the characteristics of the target strain.
• Using the selected medium and culturing the strain under the culture conditions suitable for the target bacteria,, and at the same time, repeated operations such as large-scale separation and purification are carried out to obtain the target bacteria.
• Perform strain identification on the final purified strains, such as 16S rRNA gene sequencing or 18S/ITS sequencing, to reconfirm the obtained strains at the genetic level.
• Expanded culture of purified strains according to customer needs.

Figure 1. Protocol of Strain Isolation & Culture

Technology Platforms

• High-throughput sequencing technology platform: 16S rRNA gene sequencing and 18S/ITS sequencing.
• A platform for aerobic/anaerobic isolation and culture of microbial communitie.

Why Choose Creative BioMart Microbe?

• Multiple advanced platforms to support your different purposes to isolate bacteria or fungi
• Standardized management system and optimized experimental procedures to ensure efficient and accurate test results
• Customized strategy to meet different customer's research needs
• Timely feedback and communication to ensure accurate execution and reorientation of protocols
• One-stop service to accelerate the research process

Creative BioMart Microbe supports strain isolation and culture services through multiple biotechnology platforms. If you have relevant research needs or want to know more about our services, please feel free to contact us.