DNA Fingerprinting Service

DNA fingerprinting is an electrophoretic map of DNA that identifies differences between individuals, based on the molecular markers of DNA. This electrophoretic map is rich in polymorphism, has high individual specificity and environmental stability, just like human fingerprints, so it is called "fingerprint". Fingerprint is a powerful tool for identifying varieties and strains, and it has the advantages of being fast and accurate. 16S rDNA is common in prokaryotes, and contains relatively conserved and variable regions, and the size is suitable for electrophoretic analysis. Fingerprint analysis based on 16S rDNA gene has been widely used in the study of soil microbial diversity, which can determine the phylogenetic relationship of specific microorganisms.

DNA Fingerprinting Service

Molecular biology techniques based on 16S rDNA gene fingerprint analysis have been developed rapidly. These techniques overcome the limitations of traditional culture techniques, provide a method to analyze the entire microbial community, and more accurately reveal the microbial population structure and dynamic changes. Creative BioMart Microbe provides several strategies to accelerate the process of microbial DNA fingerprinting.

• Denaturing Gradient Gel Electrophoresis and Temperature Gradient Gel Electrophoresis

Denaturing gradient gel electrophoresis (DGGE) is widely used to compare the diversity of microbial communities in different ecosystems and to monitor the dynamic changes of specific microbial populations. Temperature gradient gel electrophoresis (TGGE) method combined with PCR amplification of marker genes or their transcripts (rRNA and mRNA) can directly reveal the dominant components of microbial communities.

• Randomly Amplified Polymorphic DNA

Randomly amplified polymorphic DNA technique (RAPD) uses random oligonucleotides (5-10 nt) as primer, and the polymorphic fragments obtained by amplification are shorter, which is more convenient to detect the existence of two homologous or heterologous alleles, and is suitable for more detailed classification within species and subspecies.

• Restriction Fragment Length Polymorphism and Amplified Ribosomal DNA Restriction Analysis

Restriction fragment length polymorphism (RFLP) method and amplified ribosomal DNA restriction analysis (ARDRA) are also commonly used to analyze microbial communities. The 16SrDNA fragment was amplified by PCR with universal primers, cleaved by restriction enzyme, and then analyzed by electrophoresis to form fragments of different lengths.

Figure 1. Dendrogram derived from the UPGMA linkage of Pearson correlation coefficients of combined fingerprinting data sets for all Brettanomyces strains (Crauwels S.; et al. 2014)

• One-dimensional TRFLP-SSCP

One-dimensional TRFLP-SSCP is an improved DNA fingerprinting technique that can simultaneously distinguish between broad taxonomic branches based on terminal fragment length polymorphisms (T-RFLP) and closely related sequences based on single-strand conformation polymorphisms (SSCP).

Why Choose Creative BioMart Microbe?

• Mature research and development technology platform
• Adequate stock reserve
• Detailed and reliable test report
• Strong technical service ability

Workflow of DNA Fingerprinting Service

Creative BioMart Microbe is committed to providing customers with efficient and high-quality DNA fingerprinting services through a comprehensive technical platform, and personalized customized services can meet the research needs of different customers. Please feel free to contact us for more detailed service information.

Reference:

1. Crauwels, S.; et al. Assessing genetic diversity among Brettanomyces yeasts by DNA fingerprinting and whole-genome sequencing. Appl Environ Microbiol. 2014, 80(14): 4398-4413.