Evaluation of Probiotic Properties in vitro

Probiotics are defined as live microganisms, which when administered in adequate amounts, confer a health benefit on the host. It plays an important role in regulating intestinal microbiota, promoting the absorption of nutrients, stabilizing the integrity of intestinal mucosa, defending the pathogenic bacteria and enhancing immunity. It has been widely used in food, dairy products, health products, medicine, agriculture and other fields. As probiotics, the following characteristics are needed: the resistance to gastrointestinal tract, the ability to colonize the host intestine, the inhibition of pathogen growth and the safety. To evaluate the potential probiotic properties and the safety in vitro is of great significance to improve the quality, the efficacy of probiotics and the health.

Creative BioMart Microbe offers the evaluation of probiotic properties in vitro, providing a theoretical basis for the development and application of strains in medicine, food, biology and other related fields.

In vitro resistance to simulated gastrointestinal juices

Evaluating the resistance to the extreme conditions of the GI tract is a necessary step for the selection of probiotic strain. As an excellent probiotic solution provider, Creative BioMart Microbe has extensive experience in probiotic services. we offer the superior quality solutions by exposing strains to simulated gastrointestinal tract, and a more accurate prediction of the resistance to gastrointestinal tract can be obtained. Our service covers:

• Resistance to simulated gastric juice solutions
• Resistance to pancreatin solutions
• Resistance to bile salt solutions

Cell surface characteristics

Adhering to the intestinal cells is also an important property, which means that they can stay in the intestine for a long time and benefit the host. The autoaggregation ability of probiotics is positively related to its adhesion, which can be used as one of the indexes to screen probiotics. The coaggregation between probiotics and pathogens can interfere the ability of the pathogens to adhere to epithelial surface. The surface hydrophobicity is also a factor affecting adhesion. Creative BioMart Microbe is dedicated to analyze cell surface characteristics services including:

• The Auto-aggregation assay of probiotics
• The Coaggregation between probiotics and pathogens assay
• The Cell Surface Hydrophobicity analysis

Antibacterial activity

Probiotics produce active substances such as lactic acid, bacteriocin and hydrogen peroxide in the process of metabolism, which can inhibit the growth of pathogenic microorganism, thus regulating the balance of intestinal flora, preventing diarrhea or relieving corresponding symptoms, so as to benefit the host. Creative BioMart Microbe has established a platform to analyze the antibacterial activity of probiotics against opportunistic microorganisms like Escherichia coli, Staphylococcus aureus and Salmonella, assisting our clients to better select potential probiotic strains.

The safety evaluation of probiotics

Besides the basic criteria for the selection of probiotics, the safety evaluation of probiotics must be performed before its application. Creative BioMart Microbe is dedicated to safety evaluation, the service including:

• The evaluation of resistance to antibiotics
• The blood hemolytic activity analysis
• In vitro cytotoxicity assay

Research objects

Animal Bifidobacterium, Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus rhamnosus, Streptococcus thermophilus, Bacillus coagulans, etc.

Why Choose Creative BioMart Microbe?

• Excellent team with a multidisciplinary background.
• Fast, stable, and complete evaluation of probiotics in vitro.
• The experienced technical team always ensures the implementation of strict and standardized experimental procedures to ensure the reliability and repeatability of the results.
• Short service cycle and competitive price.
• Urgent services are available.
• Ensure 24/7 online service.

Workflow of Agricultural Pathogen Detection Service in Creative BioMart Microbe

We are committed to providing a reliable theoretical basis for the project with our evaluation service, advancing the project and helping our clients make the best choice. If you are interested in our evaluation of probiotic properties in vitro services, please feel free to contact us. Creative BioMart Microbe looks forward to cooperating with you.