Bacteriocin is safe and not easy to develop drug resistance. High purity bacterial vegetarianism is an important basis for the study of molecular and genetic characteristics of bacteriocin, so the separation and purification of bacteriocin is of great significance and has become a research hotspot.
Figure 1. Bacteriocin layout from its production to characterization
There are three major strategies in Creative BioMart Microbe for the complete purification of bacteriocin, these are, conventional multi-step method, simple three-step method and single-step bed adsorption. The precipitation, ion exchange chromatography and reversed-phase chromatography are the main techniques used. All purification possibilities depend on the nature of bacteriocin and differ by the duration of purification process, the recovery and yield of bacteriocin and target of the analysis.
Figure 2. Possible ways for bacteriocin purification
Why Choose Creative BioMart Microbe?
- There are a variety of bacteriocin purification technologies, such as ammonium sulfate in different concentrations, cation exchange chromatography (IEC) and reverse phase high performance liquid chromatography (RP-HPLC) purification.
- Has short and quality testing period, low cost, complete experimental scheme, and scientific, fair and accurate testing report issued. Bacteriocin can be purified by a several-step purification method and its molecular weight was determined by electrophoresis (SDS-PAGE/ Tricine-SDS-PAGE) and AA sequencing.
- A number of laboratory branches, support door-to-door sampling/sample sent testing services!
- All experiments sign confidentiality agreements and pay attention to the protection of customer privacy.
- Ensure 24/7 online service.
Workflow of Bacteriocin Purification at Creative BioMart Microbe
Our laboratory keeps a close eye on the development of science and technology and uses the latest test equipment and methods. It also provides other measurement, testing and analysis services as well as antimicrobial activity assays. Creative BioMart Microbe is your best partner. If you are interested in our agricultural pathogen testing services, please feel free to contact us. Creative BioMart Microbe looks forward to cooperating with you.
Reference
- Sharma, V.; et al. Bacteriocins: Production, different strategies of purification and applications.International Journal of Research in Pharmaceutical Sciences. 2019, 10(3): 1808-1817.
