Construction of In Vitro Biofilm Models
Summary of the Construction of In Vitro Biofilm Models
Most of our understanding of the physiology and microecology of biofilms comes from the study of in vitro biofilm models. In vitro biofilm models are widely used in various fields. Compared with animal models, in vitro biofilm models are simple to operate, inexpensive, high throughput, and are usually used to further test promising candidates obtained from the use of in vitro models.
In vitro models do not have the host immune system like in vivo models, and other important differences such as lower oxygen availability in many in vivo conditions are usually unaddressed. In vitro biofilm models usually include glass slides, Petri dishes, and more detailed systems that consider the mechanical and physicochemical properties required to replicate biofilm formation in a specific condition.
Complex in vitro biofilm models can also consider the cellular interaction between cells and the incorporation of monitoring systems for real-time assessment of biofilm formation. According to the availability of nutrients, in vitro biofilm models can be divided into two groups:
(1) Closed models (static models): Oxygen and nutrients supply are limited to the initially provided conditions, or periodically renewed after the waste is removed;
(2) Open models (dynamic models): A continuous nutrient supply and waste removal over time are provided. In addition, the traditional in vitro biofilm models can also be improved by converting them to microcosm models by including cells and/or substances normally found under simulated conditions.
The Construction of In Vitro Biofilm Models Provided by Creative BioMart Microbe
Creative BioMart Microbe has accumulated a lot of experience in the construction of in vitro biofilm models, and has established a powerful and advanced biofilm model construction platform. It provides microtiter plate, drip flow reactor, multiplaque artificial mouth, trickle reactors, and Calgary biofilm device (CBD), rotating disk reactor, colony biofilm model, CDC biofilm reactor, microfluidic biochips, BioFlux and other equipment.
Figure 1. The drip flow biofilm fermenter (McBain, A.J. 2009)
At the same time, we continue to upgrade our technology platform and introduce a large number of new technologies to support the construction of in vitro biofilm models. We provide various advanced technologies including 3D bioprinting, impedance-based multielectrode array, biofilm rheometer plate, microcalorimetry flow system, microfluidic agarose channel device, microfluidic wound model, etc., on our platform, we can solve all the challenges encountered in the project for customers.
Although every technology or device has undergone long-term development and improvement, they all have inherent shortcomings and limitations. Customers can consult our microbiology experts for free, and we will provide you with customized experimental programs.
As an outstanding microbiological research institution, Creative BioMart Microbe provides high-quality construction of in vitro biofilm models to help customers research biofilms more efficiently. Our excellent technology platform can realize a variety of biofilm technical services, the praise from customers all over the world is the proof of our quality service.
If you want to know more about this service, please feel free to contact us by phone or email, or you can consult through our inquiry form. We will ensure to provide you with efficient and high-quality experimental outsourcing services.
References
- Guzmán-Soto, I.; et al. Mimicking biofilm formation and development: Recent progress in in vitro and in vivo biofilm models. iScience. 2021, 5.
- McBain, A.J. In Vitro Biofilm Models. Advances in Applied Microbiology. 2009, 69: 99-132.
