Identification of Bacteria in Biofilm

Summary of the Identification of Bacteria in Biofilm

Due to the complex and the heterogeneous biofilm matrix, the identification of infections caused by biofilms is extremely challenging. Infections caused by biofilms are difficult to cure because the biofilm matrix provides bacteria physical protection from antibiotic treatment. In medicine, the rapid detection and identification of biofilms is particularly important. It can provide key information for the early diagnosis and effective treatment of infections.

However, due to its inherent physiological heterogeneity, the identification of bacterial species in biofilms becomes difficult. Traditional biofilm detection methods usually require several days, because these methods rely on culturing and have low sensitivity. Therefore, accurate and rapid identification methods are urgently needed to promote our understanding of biofilms.

The Identification of Bacteria in Biofilm Provided by Creative BioMart Microbe  

In order to help customers efficiently identify bacteria in biofilms and fully study biofilms, Creative BioMart Microbe has introduced a variety of advanced technologies to form a professional technology platform. In addition to molecular detection methods such as fluorescence in situ hybridization (FISH) and PCR and other technologies, we also provide rapid detection technologies newly developed in recent years, including:

• The gold nanoparticle (AuNP)-based multichannel sensor can identify bacterial species and even strains in biofilms based on the physicochemical properties of biofilms. This technology has been used to successfully identify and distinguish the biofilm formed by pathogenic and nonpathogenic bacteria

Figure 1. Schematic illustration of the multichannel sensor (Li, X.N.; et al. 2014)

• The polymer-based sensor array can identify and distinguish bacterial species based on the selective interaction between the biofilm matrix and the polymer sensor element. This selective multivalent interaction can lead to differences in fluorescence patterns, thereby providing the characteristics of biofilm species.

Figure 2. Molecular design and working principle of the polymer sensor platform (Ngernpimai, S.; et al. 2019)

Creative BioMart Microbe has accumulated a lot of experience in biofilm research and introduced different traditional and novel identification tools. They all have the potential to become effective diagnostics and provide a way for the rapid diagnosis of biofilm infections. Customers can consult our microbiology experts for free, and they can customize the experimental program according to your needs.

If you want to know more about this service, please feel free to contact us by phone or email, or you can consult through our inquiry form. We will ensure to provide you with efficient and high-quality experimental outsourcing services.

References

1. Li, X.N.; et al. Rapid Identification of Bacterial Biofilms and Biofilm Wound Models Using a Multichannel Nanosensor. ACS Nano. 2014.
2. Ngernpimai, S.; et al. Rapid Identification of Biofilms Using a Robust Multichannel Polymer Sensor Array. ACS Appl. Mater. Interfaces. 2019, 11: 11202-11208.