Bacterial Plasmids Isolation Service
Isolating plasmids DNA from bacteria is an important technical method in biological research, especially in genetic engineering research. Creative BioMart Microbe’s technical experts have rich project experience and can help customers isolate high-quality plasmids from bacterial samples to make your research easier.
Plasmid
Plasmids are circular or linear double-stranded DNA molecules that exist in many bacteria and archaea. Plasmids are independent of chromosome replication and are usually much smaller than chromosomes. Although most plasmids carry non-essential genes, they carry genes that can profoundly affect the physiology of the host cell. More than 300 different plasmids have been isolated from E. coli strains alone. Most plasmids are circular, about 1kbp-1Mbp in size, and exist in the cytoplasm as free DNA.
The most widely studied plasmid is the resistance plasmid, which can make people resistant to antibiotics. Bacteria that are resistant to antibiotics play an important role in medical research. Plasmid genes may also encode some special metabolic enzymes. In addition, plasmids also play a key role in the horizontal gene transfer process of bacteria. Plasmid DNA can be used for downstream applications such as transfection, screening clones, sequencing, and restriction digestion.
The Bacterial Plasmids Isolation Service
Isolation of plasmid DNA from bacteria includes three steps: culturing bacteria to amplify plasmids, collecting and lysing cells, and isolating and purifying plasmid DNA. In the process of cell lysis, it is necessary to use biological and chemical reagents to destroy the cell wall and lyse the cell membrane so that the plasmid DNA can come out of the cell. When using strong heat or acid or alkali treatment, the chromosomal DNA of the bacteria is denatured and then entangled and attached to the cell debris. The plasmid DNA will re-form natural supercoiled molecules after the external conditions are restored, and will exist in the liquid phase in a dissolved state. Thus, the plasmid DNA can be separated from the lysate by physical methods such as centrifugation.
Why Choose Creative BioMart Microbe?
- Achieve the extraction of plasmid DNA from a large number of samples and obtain high-yield plasmid DNA
- Separation and purification services for plasmid DNA of different scales
- The standardized operation to avoid DNA contamination
- Submit detailed experiment data and reports
- High-efficiency service and competitive price
- Ensure 24/7 online service.
Workflow of Bacterial Plasmids Isolation Service in Creative BioMart Microbe
Creative BioMart Microbe provides customers with high-quality and high-efficiency microbiological-related technical services, a professional technical team, and a strict quality management system to ensure that we provide customers with accurate and reliable experimental results.
If you want to know more about this service, please feel free to contact us by phone or email, or you can consult through our inquiry form. We will ensure to provide you with efficient and high-quality experimental outsourcing services.
