Biofilm Forming Ability Test

Summary of the Biofilm Forming Ability Test

Biofilm, as a structured community where aerobic or anaerobic microorganisms adhere to each other and to an inert or biological surface, plays an important role in all aspects. In the natural environment and the human host, bacteria will exhibit in two forms: mobile cells and cell-forming biofilms, the latter being the most common mode. Since biofilms are widely distributed, they can contaminate various surfaces, including food contact surfaces, dental plaque, medical devices, aquatic systems, etc. According to statistics, more than 75% of all infections are caused by biofilms. Multiple studies have also shown that the concentration of antibiotics required to inhibit bacteria within biofilm may be 1,000 times the concentration used to inhibit the same bacteria in planktonic states.

Therefore, the study of microbial biofilms is of great significance. It can not only understand the microbial diversity in biofilms, but also study its impact on various fields, or use biofilm-forming microbes to evaluate the anti-biofilm-forming activities of certain reagents and molecules.

Figure 1. Confocal microscopy images of biofilm from S61 and biofilm from S. aureus (Jardak, M.; et al. 2017)

The Biofilm Forming Ability Test Provided by Creative BioMart Microbe

Creative BioMart Microbe has always insisted on conducting in-depth research on biofilms, and recruited a large number of microbiological experts to form a professional team. We aim to solve all the problems encountered in the process of researching microorganisms for customers, and provide various biofilm technical services including biofilm forming ability testing. The biofilm forming ability test we provide includes but is not limited to the following:

Preliminary Tests

• Motility test: Illustrate the bacterial motility by measuring the migration distance in the Petri dishes.
• Microbial adhesion to solvents (MATS): Study the physicochemical properties of the cell surface.
• CRA method: Evaluate the slime production of the isolates by the Congo Red Agar Method (CRA).

In Vitro Tests

• Crystal violet test: Detect the attachment of microorganisms to abiotic surfaces.
• Resazurin test: Test the viability of biofilms.

Confocal microscopy

Visualization of biofilms using confocal scanning microscopy.

As an outstanding microbiological research institution, Creative BioMart Microbe provides high-quality biofilm forming ability test and techniques to help customers research biofilms more efficiently. Our excellent technology platform can realize a variety of biofilm technical services, and solve all the difficulties encountered in research for customers. The praise from customers all over the world is the proof of our quality service.

If you want to know more about this service, please feel free to contact us by phone or email, or you can consult through our inquiry form. We will ensure to provide you with efficient and high-quality experimental outsourcing services.

Reference

1. Jardak, M.; et al. Evaluation of biofilm-forming ability of bacterial strains isolated from the roof of an old house. J. Gen. Appl. Microbiol. 2017, 63: 186-194.