Cat.No.
TRR1-1907Y
Species
Saccharomyces cerevisiae
Product Name
Recombinant Yeast TRR1
Product Overview
Recombinant Yeast Thioreduxin Reductase produced inE.coliis a single, non-glycosylated, polypeptide chain, 36kDa.
Source
E. coli
Form
Purified, in PBS, pH 7.4.
Purity
>95%, as determined by: (a) Analysis by RP-HPLC and anion-exchange FPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Storage
-20°C (aliquot), avoid freezing and thawing cycles.
Synonyms
Cytoplasmic thioredoxin reductase, key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; TRR1;Cytoplasmic thioredoxin reductase, key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress Trr1p
Endotoxin
Less than 0.1 ng/µg (IEU/µg) of ryNTR.
Gene ID
Pathway
Pyrimidine metabolism
Protein Refseq
Chromosome Location
IV
Function
FAD binding; ferrous iron binding; oxidoreductase activity; thioredoxin-disulfide reductase activity
Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Lys-Ala-Met-His-Val.
Biological Activity
RyNTR is fully biologically active when compared to standardt Definition: One unit equals the change in absorbance at 412 nm per ute at 25°C using 0.2mM NADPH containing 5mM DTNB (pH 7.0) specific activity was found to be 5IU/mg.
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Lys-Ala-Met-His-Val.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Lys-Ala-Met-His-Val.
Dimers & Aggregates
Less than 1% as determined by silver stained SDS-PAGE gel analysis.
Protein Content
Protein quantitation was carried out by two independent methods: 1) UV spectroscopy at 280 nm. 2) Analysis by RP-HPLC, using a standard solution of CTLA-4 as a Reference Standard.
Application
1) Molecular standard (Western, ELISA) in studying secreted BMP-4. 2) Preparing antibodies for BMP-4 monomer. 3) Molecule standard in detecting secreted BMP-4 in reduced SDS-PAGE.
Pathways
Pyrimidine metabolism
