Cat.No.
MET3-26Y
Species
Yeast
Product Name
Recombinant Yeast MET3 Protein, His-tagged
Product Overview
Recombinant Yeast MET3 Protein (Pro2-Phe511) is produced by E. coli-derived expression system. This protein is fused with N-terminal Met and 6-His tag
Description
Sulfur metabolism is essential to all organisms. Inorganic sulfate is sequentially activated by ATP Sulfurylase and adenosine 5′-phosphosulfate kinase (APSK). ATP Sulfurylase converts ATP to adenosine-5'-phosphosulfate (APS) and generates pyrophosphate. APSK subsequently phosphorylates APS at the 3’ site to generate 3'‑phosphoadenosine-5'-phosphosulfate (PAPS). PAPS is the sulfur donor required for all sulfotransferase reactions in humans and is also the sulfur source for synthesis of cysteine, methionine and glutathione. The ATP Sulfurylase reaction is highly unfavorable energetically with the standard free-energy change of approximately +46 kJ/mol. To drive the reaction forward, APSK and pyrophosphatase are generally added into the reaction simultaneously to remove the products. The recombinant S. cerevisiae ATP Sulfurylase can be used for in vitro PAPS synthesis.
Source
E. coli
Tag
His
Form
Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol and DTT.
Molecular Mass
59 kDa
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Storage
Avoid repeated freeze-thaw cycles.
6 months from date of receipt, -70 centigrade as supplied.
3 months, -70 centigrade under sterile conditions after opening.
6 months from date of receipt, -70 centigrade as supplied.
3 months, -70 centigrade under sterile conditions after opening.
UniProt ID
Protein length
Pro2-Phe511
Bio-activity
Measured by its ability to sulfurylate ATP. The specific activity is >2,500 pmol/min/μg.
Endotoxin
<1.0 EU per 1 μg of the protein by the LAL method.
Predicted N Terminal
Met
