Cat.No.
LMA1-98Y
Species
Saccharomyces cerevisiae
Product Name
Recombinant Yeast LMA1
Product Overview
Recombinant yeast Thioredoxin produced inE.Coliis a single, non-glycosylated, polypeptide chain and has a molecular mass of 12.6kDa.
Description
Cytoplasmic thioredoxin isoenzyme of the thioredoxin system which protects cells against oxidative and reductive stress, forms LMA1 complex with Pbi2p, acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance.
Source
E. coli
Form
Each mg of protein contains 20mM phosphate buffer pH 7.4.
Purity
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
Synonyms
LMA1; Thioredoxin; TRX1; YLR043C
UniProt ID
Endotoxin
Less than 0.1 ng/µg (IEU/µg) of ryTRX.
Gene ID
Protein Refseq
Chromosome Location
XII
Stability
RyTRX although stable at 4°Cfor 3 weeks, should be stored desiccated below -18°C. Please prevent freeze thaw cycles.
Physical Appearance
Sterile Lyophilized Powder.
Solubility
It is recommended to reconstitute the lyophilized ryTRX in sterile 18MΩ-cm H2O.
Formulation
Each mg of protein contains 20mM phosphate buffer pH 7.4.
Biological Activity
RyTRX is fully biologically active when compared to standard. TRX activity is assayed by measuring the change in absorbance at 650 nm at 25°C using 0.13µM bovine insulin containing 0.33mM DTT (pH 6.5). The specific activity was found to be 3IU/mg.
Dimers And Aggregates
Less than 1% as determined by silver stained SDS-PAGE gel analysis.
Dimers and aggregates
Less than 1% as determined by silver stained SDS-PAGE gel analysis.
