Cat.No.
GLC8-346Y
Species
Saccharomyces cerevisiae
Product Name
Recombinant Yeast Glc8p, His-tagged
Product Overview
Recombinant yeast GLC8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (265 aa,30.7 kDa).
Description
GLC8 is a regulatory subunit of protein phosphatase 1 (Glc7p). This protein involved in glycogen metabolism and chromosome segregation, proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2. Under normal conditions, Glc8p activates Glc7p, but when Glc8p is over produced, it may also inhibit Glc7p function. Glc8p is activated upon phosphoylation by Pho85p complexed with four cyclins (Pcl6p, Pcl7p, Pcl8p, or Pcl10p).
Source
E. coli
Form
Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 10% glycerol.
Purity
> 95% by SDS – PAGE.
Storage
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name
Synonyms
GLC8; Glc8p; GLC8 Glc8p; Regulatory subunit of protein phosphatase 1 (Glc7p), involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2.
UniProt ID
Concentration
1 mg/ml (determined by Bradford assay).
Gene ID
Protein Refseq
Chromosome Location
XIII
Function
enzyme activator activity.
Formulation
Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 10% glycerol.
Molecular Weight
30.7 kDa (265 aa).
Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMGGI LKNPLALSPE QLAQQDPETL EEFRRQVYEN TQKNAKLTSH KRNIPGLDNT KEEGEIIGTS STFLPKDTLS LKHEQDMLAK MTPEERVQWN QRNLAENEIT KKQFQDIHID EPKTPYQGAV DPHGEYYRVD DDEDEDNSDK KPCQVANDDI DDLSLGEPEF EIKENKQPDF ETNDENDEDS PEARHKKFEE MRKKHYDVRA IFNKKSREAL KDEDEDEDDS TTKEP
