Cat.No.
bga-824X
Species
X. campestris
Product Name
Recombinant X. campestris bga Protein, Met & His-tagged
Product Overview
Recombinant X. campestris bga (Accession # NP_638243) Ser25-Glu613, fused with an N-terminal Met and 6-His tag, was produced in E. coli
Source
E. coli
Tag
His
Predicted N Terminal
Met
Form
Supplied as a 0.2 µm filtered solution in Tris and NaCl.
Bio-activity
Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl-beta -D-galactopyranoside.The specific activity is >14,000 pmol/min/µg, as measured under the described conditions. See Activity Assay Protocol on www.RnDSystems.com.
Molecular Mass
Recombinant X. campestris bga, Met & His-tagged has a calculated MW of 67 kDa. In SDS-PAGE migrates as 63 kDa, reducing conditions.
Protein length
Ser25-Glu613
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
