Cat.No.
ZOT-2171V
Species
Vibrio cholerae
Product Name
Recombinant Vibrio Cholerae Serotype O1 ZOT Protein (1-399 aa), His-tagged
Product Overview
Recombinant Vibrio Cholerae Serotype O1 (strain ATCC 39315/El Tor Inaba N16961) ZOT Protein (1-399 aa) is produced by E. coli expression system. This protein is fused with a 10xHis tag at the N-terminal. Protein Description: Full Length.
Description
Increases the permeability of the small intestine mucosa by affecting the structure of intercellular tight junctions (zonula occludens)
Source
E. coli
Tag
His
Form
Tris-based buffer,50% glycerol
Molecular Mass
48.4 kDa
Protein length
1-399 aa
AA Sequence
MSIFIHHGAPGSYKTSGALWLRLLPAIKSGRHIITNVRGLNLERMAKYLKMDVSDISIEFIDTDHPDGRLTMARFWHWARKDAFLFIDECGRIWPPRLTVTNLKALDTPPDLVAEDRPESFEVAFDMHRHHGWDICLTTPNIAKVHNMIREAAEIGYRHFNRATVGLGAKFTLTTHDAANSGQMDSHALTRQVKKIPSPIFKMYASTTTGKARDTMAGTALWKDRKILFLFGMVFLMFSYSFYGLHDNPIFTGGNDATIESEQSEPQSKATVGNAVGSKAVAPASFGFCIGRLCVQDGFVTVGDERYRLVDNLDIPYRGLWATGHHIYKDTLTVFFETESGSVPTELFASSYRYKVLPLPDFNHFVVFDTFAAQALWVEVKRGLPIKTENDKKGLNSIF
Purity
> 90% as determined by SDS-PAGE.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration
A hardcopy of COA with reconstitution instruction is sent along with the products.
Synonyms
zot; Zonular occludens toxin Short name: Zot;
UniProt ID
Figure 1
ZOT-2171V, 1.jpg
Figure Note 1
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
