Cat.No.
CTXB-388V
Species
Vibrio cholerae
Product Name
Recombinant Vibrio cholerae serotype O1 CTXB protein, His-tagged
Product Overview
Recombinant Vibrio cholerae serotype O1 CTXB protein (Thr22-Asn124), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293).
Description
Cholera enterotoxin subunit B (CTXB) is also known as cholera enterotoxin B chain, cholera enterotoxin gamma chain and choleragenoid. Vibrio cholerae O1 exists as two major serotypes, Inaba and Ogawa, which are associated with the O antigen of the lipopolysaccharide and are capable of unequal reciprocal interconversion. The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. CTXB can bind five GM1 gangliosides. Also, it has no toxic activity by itself.
Source
HEK293
Tag
His
Form
Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass
The protein has a calculated MW of 13.5 kDa. The protein migrates as 18-22 kDa under reducing (R) condition (SDS-PAGE).
Protein length
103
Endotoxin
Less than 1.0 EU per ug by the LAL method.
Purity
>90% as determined by SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name
Official Symbol
Synonyms
CTXB, TOXB
Gene ID
Protein Refseq
UniProt ID
Figure 1
CTXB-388V, 1.jpg
Figure Note 1
Vibrio cholerae serotype O1 CTXB, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Figure Title 1
SDS-PAGE of CTXB-388V
