Cat.No.
TM0306-791T
Species
T. maritima
Product Name
Recombinant T. maritima TM0306 Protein, Met & His-tagged
Product Overview
Recombinant T. maritima TM0306 (Accession # NP_228118) Ile2-Glu449, fused with an N-terminal Met and 6-His tag, was produced in E. coli
Source
E. coli
Tag
His
Predicted N Terminal
Met
Form
Supplied as a 0.2 µm filtered solution in Tris and NaCl.
Bio-activity
Measured by its ability to cleave a fluorogenic substrate 4-methylumbelliferyl-alpha -L-fucopyranoside.The specific activity is >1,600 pmol/min/µg, as measured under the described conditions. See Activity Assay Protocol on www.RnDSystems.com.
Molecular Mass
Recombinant T. maritima TM0306, Met & His-tagged has a calculated MW of 53 kDa. In SDS-PAGE migrates as 43-50 kDa, reducing conditions.
Protein length
Ile2-Glu449
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution
Reconstitute at 100 ug/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Official Symbol
TM0306
Synonyms
TM0306; alpha-L-fucosidase
Gene ID
Protein Refseq
