Cat.No.
Streptavidin-5117S
Species
Streptomyces avidinii
Product Name
Recombinant Streptomyces avidinii Streptavidin
Product Overview
Recombinant Streptavidin Protein (Streptavidin) Leu 13 - Pro 159 (Accession # CAA27265) was produced in E.coli cells
Description
Streptavidin is a 60 kDa tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity for biotin. Able to bind one molecule of biotin with each subunit. Streptavidin (PI=6.0-7.5) has lower level of non-specific binding to various biological components at physiological pH than avidin (PI=7.4), resulting from its isoelectric point (PI).Streptavidin is useful in affinity chromatography, ELISA, immunohistochemistry and Western Blotting.
Source
E. coli
Tag
N/A
Predicted N Terminal
Met
Form
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Bio-activity
One unit is defined as the amount of Streptavidin required to bind 1.0 μg biotin by HABA-Avidin method.14-15 units per mg protein supplied at 1 mg/ml.
Molecular Mass
15.4 kDa. DTT-reduced Protein migrates as 15 kDa.
Endotoxin
Less than 1.0 EU per μg of the Streptavidin by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Storage
Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC-8oC); After reconstitution under sterile conditions for 1 month (4oC-8oC) or 3 months (-20oC to -70oC).
