Cat.No.
Streptavidin-390S
Species
Streptomyces avidinii
Product Name
Recombinant Streptomyces Avidinii Streptavidin, His-tagged
Product Overview
Recombinant His tagged streptavidin was expressed in E.coli and purified by using conventional chromatography techniques,17 kDa (167 aa).
Description
Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
Form
Liquid. In 20 mM Tris-HCl buffer (pH 7.5)
Purity
> 95% by SDS - PAGE
Storage
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration
1 mg/ml (determined by Bradford assay)
