Cat.No.
Streptokinase-06
Species
Streptococcus sp.
ID
3595
Product Name
Recombinant Streptokinase (SK) protein
Product Overview
Recombinant Streptokinase (SK) protein was expressed in Escherichia coli.
Description
Streptokinase is a thrombolytic agent i.e. it dissolves vascular thrombi. This potent agent is derived from the beta haemolytic streptococci and is consequently associated with the risk of anaphylaxis. Streptokinase acts by stimulating the conversion of plasminogen to plasmin. It is a proteolytic enzyme which is able to disrupt fibrin stability and production.
Source
E. coli
Form
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
Bio-activity
Fully biologically active when compared to standard. The specific activity determined by fibrining lysis in agarose plate is 8.0 × 10⁴ IU/mg.
Molecular Mass
Approximately 47.3 kDa, a single non-glycosylated polypeptide chain containing 414 amino acids.
Protein length
414
AA Sequence
IAGPEWLLDRPSVNNSQLVVSVAGTVEGTNQDISLKFFEIDLTSRPAHGGKTEQGLSPKSKLFATDSGAMPHKLEKADLLKAIQEQLIANVHSNDDYFEVIDFASDATITDRNGKVYFADKDGSVTLPIQPVQEFLLKGHVRVRPYKEKPVQNQAKSVDVEYTVQFTPLNPDDDFRPALKDTKLLKTLAIGDTITSQELLAQAQSILNKNHPGYTIYERDSSIVTHDNDIFRTILPMDQEFTYHVKNREQAYRINKKSGLNEEINNTDLISEKYYVLKKGEKPYDPFDRSHLKLFTIKYVDVNTNELLKSEQLLTASERNLDFRDLYDPRDKAKLLYNNLDAFGIMDYTLTGKVEDNHDDTNRIITVYMGKRPEGENASYHLAYDKDRYTEEEREVYSYLRYTGTPIPDNPNDK
Endotoxin
Less than 1 EU/μg of rSK as determined by LAL method.
Purity
>97% by SDS-PAGE and HPLC analysis.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
UniProt ID
Figure 1
Streptokinase-06, 1.jpg
Figure Title 1
SDS-PAGE of Streptokinase-06
