Cat.No.
PLY-1569S
Species
Streptococcus Pneumoniae Serotype 4
Product Name
Recombinant Streptococcus Pneumoniae Serotype 4 PLY Protein (2-471 aa), His-tagged
Product Overview
Recombinant Streptococcus Pneumoniae Serotype 4 (strain ATCC BAA-334/TIGR4) PLY Protein (2-471 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Full Length of Mature Protein.
Description
Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host mbranes. After binding to target mbranes, the protein undergoes a major conformation change, leading to its insertion in the host mbrane and formation of an oligomeric pore complex. Cholesterol may be required for binding to host mbranes, mbrane insertion and pore formation. Can be reversibly inactivated by oxidation.
Source
Yeast
Tag
His
Form
Tris-based buffer,50% glycerol
Molecular Mass
54.8 kDa
Protein length
2-471 aa
AA Sequence
ANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQLKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVLTPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND
Purity
> 90% as determined by SDS-PAGE.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration
A hardcopy of COA with reconstitution instruction is sent along with the products.
Synonyms
ply; Thiol-activated cytolysin;
UniProt ID
Figure 1
PLY-1569S, 1.jpg
Figure Note 1
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
