Cat.No.
RPG-263S
Species
Streptococcus sp.
ID
391721
Product Name
Recombinant Streptococcal RPG, His-tagged, Biotinylated
Product Overview
Biotinylated Protein G (Biotinylated Protein G) was labeled with biotin based on Protein G Protein, which was produced in E.coli cells.
Description
Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing specificities. It is a 65-kDa (G148 protein G) and a 58 kDa (C40 protein G) cell surface protein that has found application in purifying antibodies through its binding to the Fc region. The native molecule also binds albumin, however, because serum albumin is a major contaminant of antibody sources, the albumin binding site has been removed from this recombinant form of Protein G.
Source
E. coli
Tag
His
Form
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.
Molecular Mass
Biotinylated Protein G, fused with the polyhistidine tag at N-terminus and a single cysteine at C-terminus. DTT-reduced protein migrates as a 31 kDa polypeptide .
Endotoxin
Less than 1.0 EU per μg of the Biotinylated Protein G by the LAL method.
Purity
>95% as determined by reduced SDS-PAGE.
Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).
